Pseudomonas aeruginosa PAO1, PA0357 (mutM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0357
Name
mutM
Synonym: mutY
Synonym: fpg
Replicon chromosome
Genomic location 401131 - 401943 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249048.1
GI 15595554
Affymetrix PA0357_mutM_at
Entrez 879307
GenBank AAG03746.1
INSDC AAG03746.1
NCBI Locus Tag PA0357
protein_id(GenBank) gb|AAG03746.1|AE004473_8|gnl|PseudoCAP|PA0357
TIGR NTL03PA00358
UniParc UPI000012AB94
UniProtKB Acc Q9L7T2
UniProtKB ID FPG_PSEAE
UniRef100 UniRef100_B7V2S0
UniRef50 UniRef50_Q4K4A5
UniRef90 UniRef90_B7V2S0

Product

Feature Type CDS
Coding Frame 1
Product Name
formamidopyrimidine-DNA glycosylase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 7.84
Kyte-Doolittle Hydrophobicity Value -0.196
Molecular Weight (kDa) 30.0
Isoelectric Point (pI) 9.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 442 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000348 (571 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0357
Search term: mutM

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 16280
Genomic Position 401445
Position in ORF 315(813)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 44513
Genomic Position 401782
Position in ORF 652(813)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_04670
Mutant ID PA14NR: 28906
Genomic Position 415806
Position in ORF 515(813)
Reference 16477005

References

Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein.
Boiteux S, O'Connor TR, Laval J.
EMBO J. 1987 Oct;6(10):3177-83.
PubMed ID: 3319582
Fpg protein of Escherichia coli is a zinc finger protein whose cysteine residues have a structural and/or functional role.
O'Connor TR, Graves RJ, de Murcia G, Castaing B, Laval J.
J Biol Chem. 1993 Apr 25;268(12):9063-70.
PubMed ID: 8473347
Characterization of the GO system of Pseudomonas aeruginosa.
Oliver A, Sanchez JM, Blazquez J.
FEMS Microbiol Lett. 2002 Nov 19;217(1):31-5.
PubMed ID: 12445642