Pseudomonas aeruginosa PAO1, PA0510 (nirE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0510
Name
nirE
Synonym: nirE
Replicon chromosome
Genomic location 572583 - 573422 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249201.1
GI 15595707
Affymetrix PA0510_at
Entrez 881871
GenBank AAG03899.1
INSDC AAG03899.1
NCBI Locus Tag PA0510
protein_id(GenBank) gb|AAG03899.1|AE004488_3|gnl|PseudoCAP|PA0510
TIGR NTL03PA00511
UniParc UPI00000D4275
UniProtKB Acc G3XD80
UniProtKB ID G3XD80_PSEAE
UniRef100 UniRef100_W0YLY2
UniRef50 UniRef50_W6LVC5
UniRef90 UniRef90_V6A922

Product

Feature Type CDS
Coding Frame 1
Product Name
NirE
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.26
Kyte-Doolittle Hydrophobicity Value 0.159
Molecular Weight (kDa) 29.7
Isoelectric Point (pI) 7.09

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2YBQ TRANSFERASE 03/09/11 The x-ray structure of the SAM-dependent uroporphyrinogen III methyltransferase NirE from Pseudomonas aeruginosa in complex with SAH and uroporphyrinogen III PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
2YBO TRANSFERASE 03/08/11 The x-ray structure of the SAM-dependent uroporphyrinogen III methyltransferase NirE from Pseudomonas aeruginosa in complex with SAH PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 537 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000495 (363 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0510
Search term: nirE
Search term: NirE

References

The Pseudomonas aeruginosa nirE gene encodes the S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase required for heme d(1) biosynthesis.
Storbeck S, Walther J, Müller J, Parmar V, Schiebel HM, Kemken D, Dülcks T, Warren MJ, Layer G
FEBS J. 2009 Oct;276(20):5973-82
PubMed ID: 19754882
Gene cluster for dissimilatory nitrite reductase (nir) from Pseudomonas aeruginosa: sequencing and identification of a locus for heme d1 biosynthesis.
Kawasaki S, Arai H, Kodama T, Igarashi Y
J. Bacteriol. 1997 Jan;179(1):235-42
PubMed ID: 8982003