Pseudomonas aeruginosa PAO1, PA0516 (nirF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0516
Name
nirF
Replicon chromosome
Genomic location 576498 - 577676 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249207.1
GI 15595713
Affymetrix PA0516_nirF_at
Entrez 882198
GenBank AAG03905.1
INSDC AAG03905.1
NCBI Locus Tag PA0516
protein_id(GenBank) gb|AAG03905.1|AE004488_9|gnl|PseudoCAP|PA0516
TIGR NTL03PA00517
UniParc UPI00001301B2
UniProtKB Acc Q51480
UniProtKB ID NIRF_PSEAE
UniRef100 UniRef100_Q51480
UniRef50 UniRef50_Q51480
UniRef90 UniRef90_Q51480

Product

Feature Type CDS
Coding Frame 1
Product Name
heme d1 biosynthesis protein NirF
Product Name Confidence: Class 1
Synonyms cytochrome oxidase
Evidence for Translation
Detected using LC-MS/MS (43.34 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.62
Kyte-Doolittle Hydrophobicity Value -0.259
Molecular Weight (kDa) 43.3
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Cytoplasmic Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 69 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000494 (728 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0516
Search term: nirF

References

Sequencing and characterization of the downstream region of the genes encoding nitrite reductase and cytochrome c-551 (nirSM) from Pseudomonas aeruginosa: identification of the gene necessary for biosynthesis of heme d1.
Kawasaki S, Arai H, Igarashi Y, Kodama T
Gene 1995 Dec;167(1-2):87-91
PubMed ID: 8566817
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344