Pseudomonas aeruginosa PAO1, PA0520 (nirQ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0520
Name
nirQ
Replicon chromosome
Genomic location 580316 - 581098 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249211.1
GI 15595717
Affymetrix PA0520_nirQ_at
Entrez 882214
GenBank AAG03909.1
INSDC AAG03909.1
NCBI Locus Tag PA0520
protein_id(GenBank) gb|AAG03909.1|AE004489_1|gnl|PseudoCAP|PA0520
TIGR NTL03PA00521
UniParc UPI00001301B9
UniProtKB Acc Q51481
UniProtKB ID NIRQ_PSEAE
UniRef100 UniRef100_Q51481
UniRef50 UniRef50_Q51481
UniRef90 UniRef90_Q51481

Product

Feature Type CDS
Coding Frame 1
Product Name
regulatory protein NirQ
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.46
Kyte-Doolittle Hydrophobicity Value -0.166
Molecular Weight (kDa) 28.9
Isoelectric Point (pI) 5.19

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 188 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000504 (372 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0520
Search term: nirQ

Human Homologs

References

Interdependence of respiratory NO reduction and nitrite reduction revealed by mutagenesis of nirQ, a novel gene in the denitrification gene cluster of Pseudomonas stutzeri.
Jüngst A, Zumft WG
FEBS Lett 1992 Dec 21;314(3):308-14
PubMed ID: 1468562
Structure and ANR-dependent transcription of the nir genes for denitrification from Pseudomonas aeruginosa.
Arai H, Igarashi Y, Kodama T
Biosci Biotechnol Biochem 1994 Jul;58(7):1286-91
PubMed ID: 7765251
Expression of the nir and nor genes for denitrification of Pseudomonas aeruginosa requires a novel CRP/FNR-related transcriptional regulator, DNR, in addition to ANR.
Arai H, Igarashi Y, Kodama T
FEBS Lett 1995 Aug 28;371(1):73-6
PubMed ID: 7664887