Pseudomonas aeruginosa PAO1, PA0523 (norC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0523
Name
norC
Replicon chromosome
Genomic location 582015 - 582455 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249214.1
GI 15595720
Affymetrix PA0523_norC_at
Entrez 882200
GenBank AAG03912.1
INSDC AAG03912.1
NCBI Locus Tag PA0523
protein_id(GenBank) gb|AAG03912.1|AE004489_4|gnl|PseudoCAP|PA0523
TIGR NTL03PA00524
UniParc UPI0000165EC1
UniProtKB Acc Q59646
UniProtKB ID NORC_PSEAE
UniRef100 UniRef100_Q59646
UniRef50 UniRef50_Q52527
UniRef90 UniRef90_Q59646

Product

Feature Type CDS
Coding Frame 1
Product Name
nitric-oxide reductase subunit C
Product Name Confidence: Class 1
Synonyms cytochrome c subunit of nitric oxide reductase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (16.34 kDa, PMID:21751344)
Charge (pH 7) -1.35
Kyte-Doolittle Hydrophobicity Value -0.257
Molecular Weight (kDa) 16.3
Isoelectric Point (pI) 6.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5GUX MEMBRANE PROTEIN 08/31/16 Cytochrome c-dependent nitric oxide reductase (cNOR) from Pseudomonas aeruginosa in complex with xenon Mus musculus; Pseudomonas aeruginosa PAO1 3.3 X-RAY DIFFRACTION 99.3
3WFE IMMUNE SYSTEM/OXIDOREDUCTASE 07/18/13 Reduced and cyanide-bound cytochrome c-dependent nitric oxide reductase (cNOR) from Pseudomonas aeruginosa in complex with antibody fragment Mus musculus; Pseudomonas aeruginosa 2.49 X-RAY DIFFRACTION 99.3
5GUW MEMBRANE PROTEIN 08/31/16 Complex of Cytochrome cd1 Nitrite Reductase and Nitric Oxide Reductase in Denitrification of Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 3.2 X-RAY DIFFRACTION 99.3
3WFC IMMUNE SYSTEM/OXIDOREDUCTASE 07/18/13 Reduced and carbonmonoxide-bound cytochrome c-dependent nitric oxide reductase (cNOR) from Pseudomonas aeruginosa in complex with antibody fragment Mus musculus; Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 99.3
3WFB IMMUNE SYSTEM/OXIDOREDUCTASE 07/18/13 Reduced cytochrome c-dependent nitric oxide reductase (cNOR) from Pseudomonas aeruginosa in complex with antibody fragment Mus musculus; Pseudomonas aeruginosa 2.7 X-RAY DIFFRACTION 99.3
3WFD IMMUNE SYSTEM/OXIDOREDUCTASE 07/18/13 Reduced and acetaldoxime-bound cytochrome c-dependent nitric oxide reductase (cNOR) from Pseudomonas aeruginosa in complex with antibody fragment Mus musculus; Pseudomonas aeruginosa 2.3 X-RAY DIFFRACTION 99.3
3O0R IMMUNE SYSTEM/OXIDOREDUCTASE 07/20/10 Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment Mus musculus; Pseudomonas aeruginosa 2.7 X-RAY DIFFRACTION 99.3

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 77 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000507 (373 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0523
Search term: norC

Human Homologs

References

Purification and characterization of nitrous oxide reductase from Pseudomonas aeruginosa strain P2.
SooHoo CK, Hollocher TC
J Biol Chem 1991 Feb 5;266(4):2203-9
PubMed ID: 1899237
Extended x-ray absorption fine structure and electron paramagnetic resonance of nitrous oxide reductase from Pseudomonas aeruginosa strain P2.
SooHoo CK, Hollocher TC, Kolodziej AF, Orme-Johnson WH, Bunker G
J Biol Chem 1991 Feb 5;266(4):2210-8
PubMed ID: 1846618
The structural genes for nitric oxide reductase from Pseudomonas aeruginosa.
Arai H, Igarashi Y, Kodama T
Biochim Biophys Acta 1995 Apr 4;1261(2):279-84
PubMed ID: 7711073
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344