Pseudomonas aeruginosa PAO1, PA0763 (mucA)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA0763
|
| Name |
mucA
|
| Replicon | chromosome |
| Genomic location | 831914 - 832498 (+ strand) |
| Transposon Mutants | 1 transposon mutants in PAO1 |
Cross-References
| RefSeq | NP_249454.1 |
| GI | 15595960 |
| Affymetrix | PA0763_mucA_at |
| Entrez | 879357 |
| GenBank | AAG04152.1 |
| INSDC | AAG04152.1 |
| NCBI Locus Tag | PA0763 |
| protein_id(GenBank) | gb|AAG04152.1|AE004511_5|gnl|PseudoCAP|PA0763 |
| TIGR | NTL03PA00764 |
| UniParc | UPI000012F8D3 |
| UniProtKB Acc | P38107 |
| UniProtKB ID | MUCA_PSEAE |
| UniRef100 | UniRef100_P38107 |
| UniRef50 | UniRef50_P38107 |
| UniRef90 | UniRef90_P38107 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
anti-sigma factor MucA
|
| Synonyms | |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | -2.31 |
| Kyte-Doolittle Hydrophobicity Value | -0.315 |
| Molecular Weight (kDa) | 21.0 |
| Isoelectric Point (pI) | 5.74 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 6IN7 | TRANSCRIPTION | 10/24/18 | Crystal structure of AlgU in complex with MucA(cyto) | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.96 | X-RAY DIFFRACTION | 100.0 |
| 6IN9 | MEMBRANE PROTEIN | 10/24/18 | Crystal structure of MucB in complex with MucA(peri) | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.801 | X-RAY DIFFRACTION | 100.0 |
| 6JAU | PROTEIN BINDING | 01/25/19 | The complex structure of Pseudomonas aeruginosa MucA/MucB. | Pseudomonas aeruginosa (strain PAO1) | 1.905 | X-RAY DIFFRACTION | 100.0 |
Virulence Evidence
| Source 1 | |
| Database | VFDB : VF0091 |
| Category | Alginate regulation |
| Evidence |
|
| Host Organism | |
| Notes | |
| Reference | |
| Source 2 | |
| Database | Victors : 4227 |
| Category | Alginate regulation |
| Evidence |
ECO:0000015
mutant phenotype evidence
|
| Host Organism | Medicago sativa |
| Notes | mucA mutant attenuated for virulence in alfalfa seedling Infection model |
| Reference | 12426404 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 45 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000737 (493 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0763
Search term: mucA
Search term: anti-sigma factor MucA
|
Human Homologs
References
|
Posttranslational control of the algT (algU)-encoded sigma22 for expression of the alginate regulon in Pseudomonas aeruginosa and localization of its antagonist proteins MucA and MucB (AlgN).
Mathee K, McPherson CJ, Ohman DE
J Bacteriol 1997 Jun;179(11):3711-20
PubMed ID: 9171421
|
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
|
Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting cystic fibrosis patients.
Martin DW, Schurr MJ, Mudd MH, Govan JR, Holloway BW, Deretic V
Proc Natl Acad Sci U S A 1993 Sep 15;90(18):8377-81
PubMed ID: 8378309
|
|
Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa.
Qiu D, Eisinger VM, Rowen DW, Yu HD
Proc Natl Acad Sci U S A 2007 May 8;104(19):8107-12
PubMed ID: 17470813
|
|
The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis.
Damron FH, Qiu D, Yu HD
J Bacteriol 2009 Apr;191(7):2285-95
PubMed ID: 19168621
|
|
Gene cluster controlling conversion to alginate-overproducing phenotype in Pseudomonas aeruginosa: functional analysis in a heterologous host and role in the instability of mucoidy.
Schurr MJ, Martin DW, Mudd MH, Deretic V
J Bacteriol 1994 Jun;176(11):3375-82
PubMed ID: 8195094
|
|
Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA.
Xie ZD, Hershberger CD, Shankar S, Ye RW, Chakrabarty AM
J Bacteriol 1996 Aug;178(16):4990-6
PubMed ID: 8759865
|
|
Alginate synthesis in mucoid Pseudomonas aeruginosa: a chromosomal locus involved in control.
Fyfe JA, Govan JR
J Gen Microbiol 1980 Aug;119(2):443-50
PubMed ID: 6785378
|
|
Genetic mapping of chromosomal determinants for the production of the exopolysaccharide alginate in a Pseudomonas aeruginosa cystic fibrosis isolate.
Ohman DE, Chakrabarty AM
Infect Immun 1981 Jul;33(1):142-8
PubMed ID: 6790439
|