Pseudomonas aeruginosa PAO1, PA0852 (cbpD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0852
Name
cbpD
Replicon chromosome
Genomic location 930653 - 931822 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249543.1
GI 15596049
Affymetrix PA0852_cpbD_at
Entrez 877771
GenBank AAG04241.1
INSDC AAG04241.1
NCBI Locus Tag PA0852
protein_id(GenBank) gb|AAG04241.1|AE004520_4|gnl|PseudoCAP|PA0852
TIGR NTL03PA00853
UniParc UPI00000C518B
UniProtKB Acc Q9I589
UniProtKB ID CBPD_PSEAE
UniRef100 UniRef100_Q9I589
UniRef50 UniRef50_Q9I589
UniRef90 UniRef90_Q9I589

Product

Feature Type CDS
Coding Frame 1
Product Name
chitin-binding protein CbpD precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.57
Kyte-Doolittle Hydrophobicity Value -0.352
Molecular Weight (kDa) 41.9
Isoelectric Point (pI) 6.85

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8C5N OXIDOREDUCTASE 01/09/23 Sub-atomic resolution structure of the chitin-binding protein D (CbpD) from Pseudomonas aeruginosa Pseudomonas aeruginosa PA14 0.75 X-RAY DIFFRACTION 100.0
7SQX OXIDOREDUCTASE 11/07/21 Crystal Structure of Pseudomonas aeruginosa lytic polysaccharide monooxygenase CbpD Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 75 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000819 (307 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0852
Search term: cbpD

Human Homologs

References

Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa.
Braun P, de Groot A, Bitter W, Tommassen J
J Bacteriol 1998 Jul;180(13):3467-9
PubMed ID: 9642203
Identification of a chitin-binding protein secreted by Pseudomonas aeruginosa.
Folders J, Tommassen J, van Loon LC, Bitter W
J Bacteriol 2000 Mar;182(5):1257-63
PubMed ID: 10671445
Two genes encoding an endoglucanase and a cellulose-binding protein are clustered and co-regulated by a TTA codon in Streptomyces halstedii JM8.
Garda AL, Fernández-Abalos JM, Sánchez P, Ruiz-Arribas A, Santamaría RI
Biochem J 1997 Jun 1;324 ( Pt 2)(Pt 2):403-11
PubMed ID: 9182697
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299