Pseudomonas aeruginosa PAO1, PA0895 (aruC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0895
Name
aruC
Synonym: argD
Synonym: dapC
Synonym: astC
Replicon chromosome
Genomic location 977910 - 979130 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1

Cross-References

RefSeq NP_249586.1
GI 15596092
Affymetrix PA0895_aruC_at
Entrez 878207
GenBank AAG04284.1
INSDC AAG04284.1
NCBI Locus Tag PA0895
protein_id(GenBank) gb|AAG04284.1|AE004524_1|gnl|PseudoCAP|PA0895
TIGR NTL03PA00896
UniParc UPI00001260C7
UniProtKB Acc O30508
UniProtKB ID ARUC_PSEAE
UniRef100 UniRef100_O30508
UniRef50 UniRef50_P40732
UniRef90 UniRef90_O30508

Product

Feature Type CDS
Coding Frame 1
Product Name
N2-Succinylornithine 5-aminotransferase (SOAT) = N2-acetylornithine 5-aminotransferase (ACOAT)
Product Name Confidence: Class 1
CommentaruC is an aminotransferase gene that has dual function in arginine catabolism (as SOAT) and in arginine biosynthesis (as ACOAT)
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.40
Kyte-Doolittle Hydrophobicity Value -0.087
Molecular Weight (kDa) 43.7
Isoelectric Point (pI) 5.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 639 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000862 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0895
Search term: aruC

Human Homologs

Ensembl 86, assembly GRCh38.p7
ornithine aminotransferase [Source:HGNC Symbol;Acc:HGNC:8091]
E-value: 3.2e-60
Percent Identity: 34.6

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_22_B4
Genomic Position 978106
Position in ORF 197(1221)
Reference 15805499
Type ISlacZ/hah insertion site
Mutant ID UWGC: 21593
Genomic Position 978599
Position in ORF 690(1221)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 5216
Genomic Position 978431
Position in ORF 522(1221)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_21_C10
Genomic Position 978351
Position in ORF 442(1221)
Reference 15805499

Transposon Insertions in Orthologs

References

Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
Itoh Y
J. Bacteriol. 1997 Dec;179(23):7280-90
PubMed ID: 9393691
Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli.
Schneider BL, Kiupakis AK, Reitzer LJ
J. Bacteriol. 1998 Aug;180(16):4278-86
PubMed ID: 9696779
The dual biosynthetic capability of N-acetylornithine aminotransferase in arginine and lysine biosynthesis.
Ledwidge R, Blanchard JS
Biochemistry 1999 Mar;38(10):3019-24
PubMed ID: 10074354
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
N-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa.
Jann A, Stalon V, Wauven CV, Leisinger T, Haas D
Proc. Natl. Acad. Sci. U.S.A. 1986 Jul;83(13):4937-41
PubMed ID: 16593724