Pseudomonas aeruginosa PAO1, PA0895 (aruC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0895
Name
aruC
Synonym: argD
Synonym: dapC
Synonym: astC
Replicon chromosome
Genomic location 977910 - 979130 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249586.1
GI 15596092
Affymetrix PA0895_aruC_at
Entrez 878207
GenBank AAG04284.1
INSDC AAG04284.1
NCBI Locus Tag PA0895
protein_id(GenBank) gb|AAG04284.1|AE004524_1|gnl|PseudoCAP|PA0895
TIGR NTL03PA00896
UniParc UPI00001260C7
UniProtKB Acc O30508
UniProtKB ID ARUC_PSEAE
UniRef100 UniRef100_O30508
UniRef50 UniRef50_P40732
UniRef90 UniRef90_O30508

Product

Feature Type CDS
Coding Frame 1
Product Name
N2-Succinylornithine 5-aminotransferase (SOAT) = N2-acetylornithine 5-aminotransferase (ACOAT)
Product Name Confidence: Class 1
CommentaruC is an aminotransferase gene that has dual function in arginine catabolism (as SOAT) and in arginine biosynthesis (as ACOAT)
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.40
Kyte-Doolittle Hydrophobicity Value -0.087
Molecular Weight (kDa) 43.7
Isoelectric Point (pI) 5.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 639 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000862 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0895
Search term: aruC

Human Homologs

Ensembl 86, assembly GRCh38.p7
ornithine aminotransferase [Source:HGNC Symbol;Acc:HGNC:8091]
E-value: 3.2e-60
Percent Identity: 34.6

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 21593
Genomic Position 978599
Position in ORF 690(1221)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 5216
Genomic Position 978431
Position in ORF 522(1221)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_21_C10
Genomic Position 978351
Position in ORF 442(1221)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_22_B4
Genomic Position 978106
Position in ORF 197(1221)
Reference 15805499

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_52720
Mutant ID PA14NR: 56469
Genomic Position 4673635
Position in ORF 1175(1221)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_52720
Mutant ID PA14NR: 56790
Genomic Position 4672960
Position in ORF 500(1221)
Reference 16477005

References

Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
Itoh Y
J. Bacteriol. 1997 Dec;179(23):7280-90
PubMed ID: 9393691
Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli.
Schneider BL, Kiupakis AK, Reitzer LJ
J. Bacteriol. 1998 Aug;180(16):4278-86
PubMed ID: 9696779
The dual biosynthetic capability of N-acetylornithine aminotransferase in arginine and lysine biosynthesis.
Ledwidge R, Blanchard JS
Biochemistry 1999 Mar;38(10):3019-24
PubMed ID: 10074354
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
N-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa.
Jann A, Stalon V, Wauven CV, Leisinger T, Haas D
Proc. Natl. Acad. Sci. U.S.A. 1986 Jul;83(13):4937-41
PubMed ID: 16593724