Pseudomonas aeruginosa PAO1, PA0898 (aruD)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: astD
Replicon chromosome
Genomic location 981422 - 982888 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_249589.1
GI 15596095
Affymetrix PA0898_aruD_at
Entrez 883040
GenBank AAG04287.1
INSDC AAG04287.1
NCBI Locus Tag PA0898
protein_id(GenBank) gb|AAG04287.1|AE004524_4|gnl|PseudoCAP|PA0898
UniParc UPI0000E825EC
UniProtKB Acc O50174
UniRef100 UniRef100_O50174
UniRef50 UniRef50_O50174
UniRef90 UniRef90_O50174


Feature Type CDS
Coding Frame 1
Product Name
N-Succinylglutamate 5-semialdehyde dehydrogenase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -4.73
Kyte-Doolittle Hydrophobicity Value 0.100
Molecular Weight (kDa) 51.5
Isoelectric Point (pI) 6.19

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3JU8 OXIDOREDUCTASE 09/14/09 Crystal Structure of Succinylglutamic Semialdehyde Dehydrogenase from Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 1.82 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 599 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000865 (541 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0898
Search term: aruD

Human Homologs

Ensembl 86, assembly GRCh38.p7
aldehyde dehydrogenase 8 family member A1 [Source:HGNC Symbol;Acc:HGNC:15471]
E-value: 3.5e-45
Percent Identity: 29.6

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 3997
Genomic Position 982106
Position in ORF 685(1467)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_44_B7
Genomic Position 982247
Position in ORF 826(1467)
Reference 15805499
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 51929
Genomic Position 981521
Position in ORF 100(1467)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_21_E8
Genomic Position 982750
Position in ORF 1329(1467)
Reference 15805499
Type ISphoA/hah insertion site
Mutant ID UWGC: 53270
Genomic Position 982847
Position in ORF 1426(1467)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_52670
Mutant ID PA14NR: 31424
Genomic Position 4670153
Position in ORF 1451(1467)
Reference 16477005


Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
Itoh Y.
J Bacteriol. 1997 Dec;179(23):7280-90.
PubMed ID: 9393691
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM.
Proteomics. 2009 Apr;9(7):1901-15.
PubMed ID: 19333994
N-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa.
Jann A(1), Stalon V, Wauven CV, Leisinger T, Haas D.
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4937-41.
PubMed ID: 16593724