Pseudomonas aeruginosa PAO1, PA0898 (aruD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0898
Name
aruD
Synonym: astD
Replicon chromosome
Genomic location 981422 - 982888 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1

Cross-References

RefSeq NP_249589.1
GI 15596095
Affymetrix PA0898_aruD_at
Entrez 883040
GenBank AAG04287.1
INSDC AAG04287.1
NCBI Locus Tag PA0898
protein_id(GenBank) gb|AAG04287.1|AE004524_4|gnl|PseudoCAP|PA0898
TIGR NTL03PA00899
UniParc UPI0000E825EC
UniProtKB Acc O50174
UniProtKB ID ASTD_PSEAE
UniRef100 UniRef100_O50174
UniRef50 UniRef50_O50174
UniRef90 UniRef90_O50174

Product

Feature Type CDS
Coding Frame 1
Product Name
N-Succinylglutamate 5-semialdehyde dehydrogenase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -4.73
Kyte-Doolittle Hydrophobicity Value 0.100
Molecular Weight (kDa) 51.5
Isoelectric Point (pI) 6.19

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3JU8 OXIDOREDUCTASE 09/14/09 Crystal Structure of Succinylglutamic Semialdehyde Dehydrogenase from Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 1.82 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 599 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000865 (531 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0898
Search term: aruD

Human Homologs

Ensembl 86, assembly GRCh38.p7
aldehyde dehydrogenase 8 family member A1 [Source:HGNC Symbol;Acc:HGNC:15471]
E-value: 3.5e-45
Percent Identity: 29.6

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 51929
Genomic Position 981521
Position in ORF 100(1467)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_44_B7
Genomic Position 982247
Position in ORF 826(1467)
Reference 15805499
Type ISphoA/hah insertion site
Mutant ID UWGC: 53270
Genomic Position 982847
Position in ORF 1426(1467)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_21_E8
Genomic Position 982750
Position in ORF 1329(1467)
Reference 15805499
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 3997
Genomic Position 982106
Position in ORF 685(1467)
Reference 14617778

Transposon Insertions in Orthologs

References

Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
Itoh Y
J. Bacteriol. 1997 Dec;179(23):7280-90
PubMed ID: 9393691
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
N-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa.
Jann A, Stalon V, Wauven CV, Leisinger T, Haas D
Proc. Natl. Acad. Sci. U.S.A. 1986 Jul;83(13):4937-41
PubMed ID: 16593724