Pseudomonas aeruginosa PAO1, PA0899 (aruB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0899
Name
aruB
Replicon chromosome
Genomic location 982885 - 984231 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249590.1
GI 15596096
Affymetrix PA0899_aruB_at
Entrez 878588
GenBank AAG04288.1
INSDC AAG04288.1
NCBI Locus Tag PA0899
protein_id(GenBank) gb|AAG04288.1|AE004524_5|gnl|PseudoCAP|PA0899
TIGR NTL03PA00900
UniParc UPI00001261AF
UniProtKB Acc O50175
UniProtKB ID ASTB_PSEAE
UniRef100 UniRef100_B7UY35
UniRef50 UniRef50_Q2SKP0
UniRef90 UniRef90_A6VA75

Product

Feature Type CDS
Coding Frame 1
Product Name
N2-Succinylarginine dihydrolase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -6.62
Kyte-Doolittle Hydrophobicity Value -0.248
Molecular Weight (kDa) 48.9
Isoelectric Point (pI) 6.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 65 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000866 (542 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0899
Search term: aruB

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_41_G8
Genomic Position 983599
Position in ORF 715(1347)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_25_H12
Genomic Position 983586
Position in ORF 702(1347)
Reference 15805499
Type ISlacZ/hah insertion site
Mutant ID UWGC: 54
Genomic Position 983911
Position in ORF 1027(1347)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 1460
Genomic Position 983575
Position in ORF 691(1347)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_37_F1
Genomic Position 983195
Position in ORF 311(1347)
Reference 15805499

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_52660
Mutant ID PA14NR: 24629
Genomic Position 4668151
Position in ORF 792(1347)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_52660
Mutant ID PA14NR: 32331
Genomic Position 4668489
Position in ORF 1130(1347)
Reference 16477005

References

Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
Itoh Y.
J Bacteriol. 1997 Dec;179(23):7280-90.
PubMed ID: 9393691
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM.
Proteomics. 2009 Apr;9(7):1901-15.
PubMed ID: 19333994
N-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa.
Jann A(1), Stalon V, Wauven CV, Leisinger T, Haas D.
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4937-41.
PubMed ID: 16593724