Pseudomonas aeruginosa PAO1, PA0901 (aruE)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: astE
Replicon chromosome
Genomic location 984549 - 985547 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_249592.1
GI 15596098
Affymetrix PA0901_aruE_at
Entrez 878165
GenBank AAG04290.1
INSDC AAG04290.1
NCBI Locus Tag PA0901
protein_id(GenBank) gb|AAG04290.1|AE004524_7|gnl|PseudoCAP|PA0901
UniParc UPI00001261B1
UniProtKB Acc O50177
UniRef100 UniRef100_B7UY33
UniRef50 UniRef50_B7UY33
UniRef90 UniRef90_B7UY33


Feature Type CDS
Coding Frame 1
Product Name
N-Succinylglutamate desuccinylase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.57
Kyte-Doolittle Hydrophobicity Value -0.266
Molecular Weight (kDa) 36.9
Isoelectric Point (pI) 6.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 48 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000868 (552 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0901
Search term: aruE

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 18006
Genomic Position 984989
Position in ORF 441(999)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_76_E6
Genomic Position 984691
Position in ORF 143(999)
Reference 15805499
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 3689
Genomic Position 985368
Position in ORF 820(999)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_74_H6
Genomic Position 985254
Position in ORF 706(999)
Reference 15805499

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_52630
Mutant ID PA14NR: 30633
Genomic Position 4666432
Position in ORF 389(999)
Reference 16477005


Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
Itoh Y
J. Bacteriol. 1997 Dec;179(23):7280-90
PubMed ID: 9393691
N-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa.
Jann A, Stalon V, Wauven CV, Leisinger T, Haas D
Proc. Natl. Acad. Sci. U.S.A. 1986 Jul;83(13):4937-41
PubMed ID: 16593724