Pseudomonas aeruginosa PAO1, PA0901 (aruE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0901
Name
aruE
Synonym: astE
Replicon chromosome
Genomic location 984549 - 985547 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249592.1
GI 15596098
Affymetrix PA0901_aruE_at
Entrez 878165
GenBank AAG04290.1
INSDC AAG04290.1
NCBI Locus Tag PA0901
protein_id(GenBank) gb|AAG04290.1|AE004524_7|gnl|PseudoCAP|PA0901
TIGR NTL03PA00902
UniParc UPI00001261B1
UniProtKB Acc O50177
UniProtKB ID ASTE_PSEAE
UniRef100 UniRef100_B7UY33
UniRef50 UniRef50_B7UY33
UniRef90 UniRef90_B7UY33

Product

Feature Type CDS
Coding Frame 1
Product Name
N-Succinylglutamate desuccinylase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.57
Kyte-Doolittle Hydrophobicity Value -0.266
Molecular Weight (kDa) 36.9
Isoelectric Point (pI) 6.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 48 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000868 (562 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0901
Search term: aruE

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 3689
Genomic Position 985368
Position in ORF 820(999)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 18006
Genomic Position 984989
Position in ORF 441(999)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_74_H6
Genomic Position 985254
Position in ORF 706(999)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_76_E6
Genomic Position 984691
Position in ORF 143(999)
Reference 15805499

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_52630
Mutant ID PA14NR: 30633
Genomic Position 4666432
Position in ORF 389(999)
Reference 16477005

References

Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
Itoh Y.
J Bacteriol. 1997 Dec;179(23):7280-90.
PubMed ID: 9393691
N-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa.
Jann A(1), Stalon V, Wauven CV, Leisinger T, Haas D.
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4937-41.
PubMed ID: 16593724