Pseudomonas aeruginosa PAO1, PA0911 (alpE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0911
Name
alpE
Replicon chromosome
Genomic location 994699 - 995151 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249602.1
GI 15596108
Affymetrix PA0911_at
DNASU PaCD00006422
Entrez 878434
GenBank AAG04300.1
INSDC AAG04300.1
NCBI Locus Tag PA0911
protein_id(GenBank) gb|AAG04300.1|AE004525_8|gnl|PseudoCAP|PA0911
TIGR NTL03PA00912
UniParc UPI00000C51B2
UniProtKB Acc Q9I546
UniProtKB ID Q9I546_PSEAE
UniRef100 UniRef100_Q9I546
UniRef50 UniRef50_Q9I546
UniRef90 UniRef90_Q9I546

Product

Feature Type CDS
Coding Frame 1
Product Name
AlpE
Product Name Confidence: Class 1
CommentInvolved in Alp-mediated lysis
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 12.64
Kyte-Doolittle Hydrophobicity Value 0.125
Molecular Weight (kDa) 16.1
Isoelectric Point (pI) 12.14

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 2 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000877 (298 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0911
Search term: alpE
Search term: AlpE

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 53916
Genomic Position 994957
Position in ORF 259(453)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 41949
Genomic Position 994805
Position in ORF 107(453)
Reference 14617778

Transposon Insertions in Orthologs

References

A self-lysis pathway that enhances the virulence of a pathogenic bacterium.
McFarland KA, Dolben EL, LeRoux M, Kambara TK, Ramsey KM, Kirkpatrick RL, Mougous JD, Hogan DA, Dove SL
Proc. Natl. Acad. Sci. U.S.A. 2015 Jul;112(27):8433-8
PubMed ID: 26100878