Pseudomonas aeruginosa PAO1, PA0920

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 1003966 - 1006611 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_249611.1
GI 15596117
Affymetrix PA0920_at
Entrez 880933
GenBank AAG04309.1
INSDC AAG04309.1
NCBI Locus Tag PA0920
protein_id(GenBank) gb|AAG04309.1|AE004526_4|gnl|PseudoCAP|PA0920
UniParc UPI00000C51BB
UniProtKB Acc Q9I537
UniRef100 UniRef100_Q9I537
UniRef50 UniRef50_A0A0B7D7G3
UniRef90 UniRef90_Q9I537


Feature Type CDS
Coding Frame 1
Product Name
alanyl-phosphatidylglycerol synthase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 21.59
Kyte-Doolittle Hydrophobicity Value 0.484
Molecular Weight (kDa) 95.9
Isoelectric Point (pI) 9.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4V34 TRANSFERASE 10/16/14 The Structure of A-PGS from Pseudomonas aeruginosa (SeMet derivative) PSEUDOMONAS AERUGINOSA 3.1 X-RAY DIFFRACTION 99.1
4V35 TRANSFERASE 10/16/14 The Structure of A-PGS from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 99.1

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 193 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000886 (538 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0920

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 40039
Genomic Position 1005715
Position in ORF 1750(2646)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_20_G10
Genomic Position 1005413
Position in ORF 1448(2646)
Reference 15805499
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 52046
Genomic Position 1004523
Position in ORF 558(2646)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_52350
Mutant ID PA14NR: 34085
Genomic Position 4645917
Position in ORF 972(2646)
Reference 16477005


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PubMed ID: 15687295
Cloning and sequencing of a 27.8-kb nucleotide sequence of the 79 degrees-81 degrees region of the Bacillus subtilis genome containing the sspE locus.
Yamamoto H, Uchiyama S, Sekiguchi J.
DNA Res. 1996 Aug 31;3(4):257-62.
PubMed ID: 8946165
Alanyl-phosphatidylglycerol synthase: mechanism of substrate recognition during tRNA-dependent lipid modification in Pseudomonas aeruginosa.
Hebecker S(1), Arendt W, Heinemann IU, Tiefenau JH, Nimtz M, Rohde M, Söll D, Moser J.
Mol Microbiol. 2011 May;80(4):935-50.
PubMed ID: 21392131
Identification and characterization of a periplasmic aminoacyl-phosphatidylglycerol hydrolase responsible for Pseudomonas aeruginosa lipid homeostasis.
Arendt W(1), Groenewold MK, Hebecker S, Dickschat JS, Moser J.
J Biol Chem. 2013 Aug 23;288(34):24717-30.
PubMed ID: 23792962
Structures of two bacterial resistance factors mediating tRNA-dependent aminoacylation of phosphatidylglycerol with lysine or alanine.
Hebecker S(1), Krausze J(2), Hasenkampf T(1), Schneider J(1), Groenewold M(2), Reichelt J(2), Jahn D(1), Heinz DW(2), Moser J(3).
Proc Natl Acad Sci U S A. 2015 Aug 25;112(34):10691-6.
PubMed ID: 26261323