Pseudomonas aeruginosa PAO1, PA0962 (dps)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0962
Name
dps
Replicon chromosome
Genomic location 1047549 - 1048019 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentDps Protects P. aeruginosa cells from H2O2-mediated oxidative stress (PMID:36902100)

Cross-References

RefSeq NP_249653.1
GI 15596159
Affymetrix PA0962_at
Entrez 881882
GenBank AAG04351.1
INSDC AAG04351.1
NCBI Locus Tag PA0962
protein_id(GenBank) gb|AAG04351.1|AE004530_4|gnl|PseudoCAP|PA0962
TIGR NTL03PA00963
UniParc UPI00000C51E1
UniProtKB Acc Q9I4Z7
UniProtKB ID Q9I4Z7_PSEAE
UniRef100 UniRef100_W0YMY0
UniRef50 UniRef50_Q2L0H0
UniRef90 UniRef90_L8MEK7

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA-binding protein from starved cells, Dps
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.0, molecular weight:17.5, PMID:21674802)
Charge (pH 7) -7.04
Kyte-Doolittle Hydrophobicity Value -0.123
Molecular Weight (kDa) 17.5
Isoelectric Point (pI) 4.70

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8FF9 METAL BINDING PROTEIN 12/08/22 Crystal structure of Apo Dps protein (PA0962) from Pseudomonas aeruginosa (orthorhombic form) Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 100.0
8FFB METAL BINDING PROTEIN 12/08/22 Crystal structure of iron bound Dps protein (PA0962) from Pseudomonas aeruginosa (orthorhombic form) Pseudomonas aeruginosa PAO1 2.25 X-RAY DIFFRACTION 100.0
8FFA METAL BINDING PROTEIN 12/08/22 Crystal structure of Apo Dps protein (PA0962) from Pseudomonas aeruginosa (cubic form) Pseudomonas aeruginosa PAO1 2.15 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 381 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000927 (509 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0962
Search term: dps

Human Homologs

References

Metalloregulation in Bacillus subtilis: isolation and characterization of two genes differentially repressed by metal ions.
Chen L, James LP, Helmann JD
J Bacteriol 1993 Sep;175(17):5428-37
PubMed ID: 8396117
First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis.
Antelmann H, Bernhardt J, Schmid R, Mach H, Völker U, Hecker M
Electrophoresis 1997 Aug;18(8):1451-63
PubMed ID: 9298659
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
A proteome analysis of the response of a Pseudomonas aeruginosa oxyR mutant to iron limitation.
Vinckx T, Wei Q, Matthijs S, Noben JP, Daniels R, Cornelis P
Biometals 2011 Jun;24(3):523-32
PubMed ID: 21207115
Pseudomonas aeruginosa Dps (PA0962) Functions in H(2)O(2) Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity.
Rajapaksha N, Soldano A, Yao H, Donnarumma F, Kashipathy MM, Seibold S, Battaile KP, Lovell S, Rivera M
Int J Mol Sci 2023 Feb 28;24(5)
PubMed ID: 36902100