Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0973
|
Name |
oprL
Synonym: pal Synonym: excC |
Replicon | chromosome |
Genomic location | 1057400 - 1057906 (+ strand) |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_249664.1 |
GI | 15596170 |
Affymetrix | PA0973_oprL_at |
DNASU | PaCD00006446 |
Entrez | 882991 |
GenBank | AAG04362.1 |
INSDC | AAG04362.1 |
NCBI Locus Tag | PA0973 |
protein_id(GenBank) | gb|AAG04362.1|AE004530_15|gnl|PseudoCAP|PA0973 |
TIGR | NTL03PA00974 |
UniParc | UPI00000C51E3 |
UniProtKB Acc | Q9I4Z4 |
UniProtKB ID | PAL_PSEAE |
UniRef100 | UniRef100_Q9I4Z4 |
UniRef50 | UniRef50_P0A138 |
UniRef90 | UniRef90_P0A138 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Peptidoglycan associated lipoprotein OprL precursor
|
Synonyms | |
Evidence for Translation |
Detected using LC-MS/MS (17.92 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis, mass spectral analysis and protein mass mapping (PMID:21863142).
Detected using 2-DE, MALDI-TOF/MS analysis (17.93 kDa, pI 5.9, PMID:16335974)
|
Charge (pH 7) | -1.32 |
Kyte-Doolittle Hydrophobicity Value | -0.432 |
Molecular Weight (kDa) | 17.9 |
Isoelectric Point (pI) | 6.27 |
Individual Mappings | |
Additional evidence for subcellular localization |
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)
|
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 411 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000938 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0973
Search term: oprL
Search term: Peptidoglycan associated lipoprotein OprL precursor
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
Nucleotide sequence of the gene for the peptidoglycan-associated lipoprotein of Escherichia coli K12.
Chen R, Henning U
Eur J Biochem 1987 Feb 16;163(1):73-7
PubMed ID: 3545827
|
Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli.
Levengood SK, Webster RE
J Bacteriol 1989 Dec;171(12):6600-9
PubMed ID: 2687247
|
The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL).
Lazzaroni JC, Portalier R
Mol Microbiol 1992 Mar;6(6):735-42
PubMed ID: 1574003
|
Molecular and immunological characterization of OprL, the 18 kDa outer-membrane peptidoglycan-associated lipoprotein (PAL) of Pseudomonas aeruginosa.
Lim A, De Vos D, Brauns M, Mossialos D, Gaballa A, Qing D, Cornells P
Microbiology (Reading) 1997 May;143 ( Pt 5):1709-1716
PubMed ID: 9168620
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|