Pseudomonas aeruginosa PAO1, PA1024

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1024
Name
Replicon chromosome
Genomic location 1109882 - 1110868 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249715.1
GI 15596221
Affymetrix PA1024_at
Entrez 882956
GenBank AAG04413.1
INSDC AAG04413.1
NCBI Locus Tag PA1024
protein_id(GenBank) gb|AAG04413.1|AE004534_11|gnl|PseudoCAP|PA1024
TIGR NTL03PA01025
UniParc UPI00000C520C
UniProtKB Acc Q9I4V0
UniProtKB ID 2NPD_PSEAE
UniRef100 UniRef100_Q9I4V0
UniRef50 UniRef50_Q9I4V0
UniRef90 UniRef90_Q9I4V0

Product

Feature Type CDS
Coding Frame 1
Product Name
NADH:quinone reductase
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -1.46
Kyte-Doolittle Hydrophobicity Value 0.040
Molecular Weight (kDa) 34.8
Isoelectric Point (pI) 6.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2GJN OXIDOREDUCTASE 03/31/06 crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate PSEUDOMONAS AERUGINOSA PAO1 2.3 X-RAY DIFFRACTION 100.0
2GJL OXIDOREDUCTASE 03/31/06 Crystal Structure of 2-nitropropane dioxygenase PSEUDOMONAS AERUGINOSA PAO1 2.0 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 345 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000986 (396 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1024

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 35922
Genomic Position 1110282
Position in ORF 401(987)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 43996
Genomic Position 1110490
Position in ORF 609(987)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_51080
Mutant ID PA14NR: 23084
Genomic Position 4539300
Position in ORF 238(987)
Reference 16477005

References

Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base.
Ha JY, Min JY, Lee SK, Kim HS, Kim do J, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW.
J Biol Chem. 2006 Jul 7;281(27):18660-7.
PubMed ID: 16682407
Functional Annotation of a Presumed Nitronate Monoxygenase Reveals a New Class of NADH:Quinone Reductases.
Ball J(1), Salvi F(1), Gadda G(2).
J Biol Chem. 2016 Sep 30;291(40):21160-21170.
PubMed ID: 27502282