Pseudomonas aeruginosa PAO1, PA1028 (amaA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1028
Name
amaA
Synonym: amaB
Replicon chromosome
Genomic location 1114774 - 1116060 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249719.1
GI 15596225
Affymetrix PA1028_at
Entrez 880062
GenBank AAG04417.1
INSDC AAG04417.1
NCBI Locus Tag PA1028
protein_id(GenBank) gb|AAG04417.1|AE004535_4|gnl|PseudoCAP|PA1028
TIGR NTL03PA01029
UniParc UPI00000C5210
UniProtKB Acc Q9I4U6
UniProtKB ID Q9I4U6_PSEAE
UniRef100 UniRef100_K1CS31
UniRef50 UniRef50_D7I2F2
UniRef90 UniRef90_A0A0C6FET6

Product

Feature Type CDS
Coding Frame 1
Product Name
L-Pipecolate oxidase
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) 8.61
Kyte-Doolittle Hydrophobicity Value -0.061
Molecular Weight (kDa) 46.2
Isoelectric Point (pI) 8.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 282 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000990 (521 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1028
Search term: amaA

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 20635
Genomic Position 1115658
Position in ORF 885(1287)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_14_F4
Genomic Position 1115973
Position in ORF 1200(1287)
Reference 15805499
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 5031
Genomic Position 1115834
Position in ORF 1061(1287)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_51040
Mutant ID PA14NR: 33300
Genomic Position 4535066
Position in ORF 1196(1287)
Reference 16477005

References

Rat liver dimethylglycine dehydrogenase. Flavinylation of the enzyme in hepatocytes in primary culture and characterization of a cDNA clone.
Lang H, Polster M, Brandsch R
Eur. J. Biochem. 1991 Jun;198(3):793-9
PubMed ID: 1710985
Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1.
Madhuri Indurthi S, Chou HT, Lu CD
Microbiology (Reading, Engl.) 2016 May;162(5):876-88
PubMed ID: 26967762
Multiple and interconnected pathways for L-lysine catabolism in Pseudomonas putida KT2440.
Revelles O, Espinosa-Urgel M, Fuhrer T, Sauer U, Ramos JL
J. Bacteriol. 2005 Nov;187(21):7500-10
PubMed ID: 16237033