Pseudomonas aeruginosa PAO1, PA1054 (shaA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1054
Name
shaA
Synonym: phaA
Replicon chromosome
Genomic location 1142061 - 1144862 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_249745.1
GI 15596251
Affymetrix PA1054_at
Entrez 881315
GenBank AAG04443.1
INSDC AAG04443.1
NCBI Locus Tag PA1054
protein_id(GenBank) gb|AAG04443.1|AE004537_8|gnl|PseudoCAP|PA1054
TIGR NTL03PA01055
UniParc UPI00000C5228
UniProtKB Acc Q9I4S0
UniProtKB ID Q9I4S0_PSEAE
UniRef100 UniRef100_S0HZ84
UniRef50 UniRef50_Q47XI9
UniRef90 UniRef90_W0WIV0

Product

Feature Type CDS
Coding Frame 1
Product Name
ShaA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 3.72
Kyte-Doolittle Hydrophobicity Value 0.927
Molecular Weight (kDa) 99.6
Isoelectric Point (pI) 7.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 564 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001016 (1050 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1054
Search term: shaA
Search term: ShaA

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 5 [Source:HGNC Symbol;Acc:HGNC:7461]
E-value: 3.3e-41
Percent Identity: 33.6

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The pha gene cluster of Rhizobium meliloti involved in pH adaptation and symbiosis encodes a novel type of K+ efflux system.
Putnoky P, Kereszt A, Nakamura T, Endre G, Grosskopf E, Kiss P, Kondorosi A
Mol Microbiol 1998 Jun;28(6):1091-101
PubMed ID: 9680201
Characterization of a multigene-encoded sodium/hydrogen antiporter (sha) from Pseudomonas aeruginosa: its involvement in pathogenesis.
Kosono S, Haga K, Tomizawa R, Kajiyama Y, Hatano K, Takeda S, Wakai Y, Hino M, Kudo T
J Bacteriol 2005 Aug;187(15):5242-8
PubMed ID: 16030218