Pseudomonas aeruginosa PAO1, PA1195 (ddaH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1195
Name
ddaH
Replicon chromosome
Genomic location 1296334 - 1297098 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249886.1
GI 15596392
Affymetrix PA1195_at
Entrez 883112
GenBank AAG04584.1
INSDC AAG04584.1
NCBI Locus Tag PA1195
protein_id(GenBank) gb|AAG04584.1|AE004549_11|gnl|PseudoCAP|PA1195
TIGR NTL03PA01196
UniParc UPI0000033BA5
UniProtKB Acc Q9I4E3
UniProtKB ID DDAH_PSEAE
UniRef100 UniRef100_Q9I4E3
UniRef50 UniRef50_Q9I4E3
UniRef90 UniRef90_Q9I4E3

Product

Feature Type CDS
Coding Frame 1
Product Name
dimethylarginine dimethylaminohydrolase DdaH
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -6.46
Kyte-Doolittle Hydrophobicity Value -0.229
Molecular Weight (kDa) 28.5
Isoelectric Point (pI) 5.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3RHY HYDROLASE 04/12/11 Crystal structure of the dimethylarginine dimethylaminohydrolase adduct with 4-chloro-2-hydroxymethylpyridine Pseudomonas aeruginosa 2.18 X-RAY DIFFRACTION 100.0
3BPB HYDROLASE 12/18/07 Crystal structure of the dimethylarginine dimethylaminohydrolase H162G adduct with S-methyl-L-thiocitrulline Pseudomonas aeruginosa 2.81 X-RAY DIFFRACTION 99.6
1H70 HYDROLASE 06/30/01 DDAH FROM PSEUDOMONAS AERUGINOSA. C249S MUTANT COMPLEXED WITH CITRULLINE PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 99.6

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 96 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001153 (298 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1195
Search term: ddaH

Human Homologs

Ensembl 110, assembly GRCh38.p14
dimethylarginine dimethylaminohydrolase 1 [Source:HGNC Symbol;Acc:HGNC:2715]
E-value: 1.8e-26
Percent Identity: 30.8

References

Cloning and sequencing of cDNA encoding NG,NG-dimethylarginine dimethylaminohydrolase from rat kidney.
Kimoto M, Sasakawa T, Tsuji H, Miyatake S, Oka T, Nio N, Ogawa T
Biochim Biophys Acta 1997 Jan 4;1337(1):6-10
PubMed ID: 9003431
DdaR (PA1196) Regulates Expression of Dimethylarginine Dimethylaminohydrolase for the Metabolism of Methylarginines in Pseudomonas aeruginosa PAO1.
Lundgren BR, Bailey FJ, Moley G, Nomura CT
J Bacteriol 2017 Apr 15;199(8)
PubMed ID: 28167521
Characterization and manipulation of the Pseudomonas aeruginosa dimethylarginine dimethylaminohydrolase monomer--dimer equilibrium.
Plevin MJ, Magalhães BS, Harris R, Sankar A, Perkins SJ, Driscoll PC
J Mol Biol 2004 Jul 30;341(1):171-84
PubMed ID: 15312771