Pseudomonas aeruginosa PAO1, PA1253 (lhpG)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 1360698 - 1362278 (- strand)
Transposon Mutants 2 transposon mutants in PAO1


RefSeq NP_249944.1
GI 15596450
Affymetrix PA1253_at
Entrez 881455
GenBank AAG04642.1
INSDC AAG04642.1
NCBI Locus Tag PA1253
protein_id(GenBank) gb|AAG04642.1|AE004555_2|gnl|PseudoCAP|PA1253
UniParc UPI00000C52CF
UniProtKB Acc Q9I491
UniRef100 UniRef100_Q9I491
UniRef50 UniRef50_C1F341
UniRef90 UniRef90_V6AJ94


Feature Type CDS
Coding Frame 1
Product Name
alpha-ketoglutaric semialdehyde dehydrogenase, LhpG
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -3.78
Kyte-Doolittle Hydrophobicity Value -0.002
Molecular Weight (kDa) 55.4
Isoelectric Point (pI) 6.36

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 494 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001205 (806 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1253
Search term: lhpG

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 33918
Genomic Position 1361252
Position in ORF 555(1581)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 15222
Genomic Position 1361551
Position in ORF 854(1581)
Reference 14617778

Transposon Insertions in Orthologs


Glutarate semialdehyde dehydrogenase of Pseudomonas. Purification, properties, and relation to L-lysine catabolism.
Chang YF, Adams E
J. Biol. Chem. 1977 Nov;252(22):7979-86
PubMed ID: 914857
Involvement of cysteine 289 in the catalytic activity of an NADP(+)-specific fatty aldehyde dehydrogenase from Vibrio harveyi.
Vedadi M, Szittner R, Smillie L, Meighen E
Biochemistry 1995 Dec;34(51):16725-32
PubMed ID: 8527447
Characterization of the p-toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2.
Junker F, Kiewitz R, Cook AM
J. Bacteriol. 1997 Feb;179(3):919-27
PubMed ID: 9006050
Molecular characterization of LhpR in control of hydroxyproline catabolism and transport in Pseudomonas aeruginosa PAO1.
Li G, Lu CD
Microbiology (Reading, Engl.) 2016 Jul;162(7):1232-42
PubMed ID: 27145750
Identification and characterization of D-hydroxyproline dehydrogenase and Delta1-pyrroline-4-hydroxy-2-carboxylate deaminase involved in novel L-hydroxyproline metabolism of bacteria: metabolic convergent evolution.
Watanabe S, Morimoto D, Fukumori F, Shinomiya H, Nishiwaki H, Kawano-Kawada M, Sasai Y, Tozawa Y, Watanabe Y
J. Biol. Chem. 2012 Sep;287(39):32674-88
PubMed ID: 22833679