Pseudomonas aeruginosa PAO1, PA1268 (lhpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1268
Name
lhpA
Replicon chromosome
Genomic location 1377439 - 1378383 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249959.1
GI 15596465
Affymetrix PA1268_at
Entrez 881376
GenBank AAG04657.1
INSDC AAG04657.1
NCBI Locus Tag PA1268
protein_id(GenBank) gb|AAG04657.1|AE004556_7|gnl|PseudoCAP|PA1268
TIGR NTL03PA01269
UniParc UPI000003E74A
UniProtKB Acc Q9I476
UniProtKB ID 4HYPE_PSEAE
UniRef100 UniRef100_Q9I476
UniRef50 UniRef50_Q9I476
UniRef90 UniRef90_Q9I476

Product

Feature Type CDS
Coding Frame 1
Product Name
Hydroxyproline 2-epimerase, LhpA
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -7.80
Kyte-Doolittle Hydrophobicity Value -0.137
Molecular Weight (kDa) 33.5
Isoelectric Point (pI) 5.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2AZP STRUCTURAL GENOMICS, UNKNOWN FUNCTION 09/12/05 Crystal Structure of PA1268 Solved by Sulfur SAD PSEUDOMONAS AERUGINOSA 2.13 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 155 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001206 (835 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1268
Search term: lhpA

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 39879
Genomic Position 1378227
Position in ORF 789(945)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 43217
Genomic Position 1377688
Position in ORF 250(945)
Reference 14617778

Transposon Insertions in Orthologs

References

Molecular characterization of LhpR in control of hydroxyproline catabolism and transport in Pseudomonas aeruginosa PAO1.
Li G, Lu CD
Microbiology (Reading, Engl.) 2016 Jul;162(7):1232-42
PubMed ID: 27145750
Identification and characterization of trans-3-hydroxy-l-proline dehydratase and Δ(1)-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria.
Watanabe S, Tanimoto Y, Yamauchi S, Tozawa Y, Sawayama S, Watanabe Y
FEBS Open Bio 2014 ;4
PubMed ID: 24649405