Pseudomonas aeruginosa PAO1, PA1494 (muiA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1494
Name
muiA
Replicon chromosome
Genomic location 1621472 - 1623127 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250185.1
GI 15596691
Affymetrix PA1494_at
Entrez 881859
GenBank AAG04883.1
INSDC AAG04883.1
NCBI Locus Tag PA1494
protein_id(GenBank) gb|AAG04883.1|AE004578_5|gnl|PseudoCAP|PA1494
TIGR NTL03PA01495
UniParc UPI00000C53A8
UniProtKB Acc Q9I3L8
UniProtKB ID Q9I3L8_PSEAE
UniRef100 UniRef100_Q9I3L8
UniRef50 UniRef50_Q9I3L8
UniRef90 UniRef90_Q9I3L8

Product

Feature Type CDS
Coding Frame 1
Product Name
mucoidy inhibitor gene A
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -4.76
Kyte-Doolittle Hydrophobicity Value -0.452
Molecular Weight (kDa) 60.6
Isoelectric Point (pI) 5.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Periplasmic Class 1
Additional evidence for subcellular localization
Detected in a periplasmic protein preparation by spheroplasting by lysozyme and sucrose.(PMID:19333994)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 91 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001408 (300 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1494
Search term: muiA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Identification and characterization of a novel inhibitor of alginate overproduction in Pseudomonas aeruginosa.
Withers TR, Yin Y, Yu HD
Pathog Dis 2014 Mar;70(2):185-8
PubMed ID: 24115673