Pseudomonas aeruginosa PAO1, PA1510 (tplE)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: tle4
Replicon chromosome
Genomic location 1639791 - 1641500 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_250201.1
GI 15596707
Affymetrix PA1510_at
Entrez 879265
GenBank AAG04899.1
INSDC AAG04899.1
NCBI Locus Tag PA1510
protein_id(GenBank) gb|AAG04899.1|AE004579_11|gnl|PseudoCAP|PA1510
UniParc UPI00000C53B7
UniProtKB Acc Q9I3K2
UniRef100 UniRef100_Q9I3K2
UniRef50 UniRef50_Q9I3K2
UniRef90 UniRef90_Q9I3K2


Feature Type CDS
Coding Frame 1
Product Name
type 6 PGAP1-like effector, TplE
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) -14.21
Kyte-Doolittle Hydrophobicity Value -0.636
Molecular Weight (kDa) 63.3
Isoelectric Point (pI) 5.13

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4R1D HYDROLASE/HYDROLASE INHIBITOR 08/05/14 The crystal structure of Tle4-Tli4 complex PSEUDOMONAS AERUGINOSA PAO1 1.749 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003845 (318 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1510
Search term: tplE

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 18290
Genomic Position 1640979
Position in ORF 1189(1710)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 6579
Genomic Position 1641200
Position in ORF 1410(1710)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_44910
Mutant ID PA14NR: 52737
Genomic Position 4004026
Position in ORF 713(1710)
Reference 16477005


Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors.
Russell AB(1), LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD.
Nature. 2013 Apr 25;496(7446):508-12.
PubMed ID: 23552891
The Pseudomonas aeruginosa Type VI Secretion PGAP1-like Effector Induces Host Autophagy by Activating Endoplasmic Reticulum Stress.
Jiang F(1), Wang X(1), Wang B(1), Chen L(1), Zhao Z(1), Waterfield NR(2), Yang G(3), Jin Q(4).
Cell Rep. 2016 Aug 9;16(6):1502-9.
PubMed ID: 27477276