Pseudomonas aeruginosa PAO1, PA1510 (tplE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1510
Name
tplE
Synonym: tle4
Replicon chromosome
Genomic location 1639791 - 1641500 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_250201.1
GI 15596707
Affymetrix PA1510_at
Entrez 879265
GenBank AAG04899.1
INSDC AAG04899.1
NCBI Locus Tag PA1510
protein_id(GenBank) gb|AAG04899.1|AE004579_11|gnl|PseudoCAP|PA1510
TIGR NTL03PA01511
UniParc UPI00000C53B7
UniProtKB Acc Q9I3K2
UniProtKB ID Q9I3K2_PSEAE
UniRef100 UniRef100_Q9I3K2
UniRef50 UniRef50_Q9I3K2
UniRef90 UniRef90_Q9I3K2

Product

Feature Type CDS
Coding Frame 1
Product Name
type 6 PGAP1-like effector, TplE
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) -14.21
Kyte-Doolittle Hydrophobicity Value -0.636
Molecular Weight (kDa) 63.3
Isoelectric Point (pI) 5.13

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4R1D HYDROLASE/HYDROLASE INHIBITOR 08/05/14 The crystal structure of Tle4-Tli4 complex PSEUDOMONAS AERUGINOSA PAO1 1.749 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003845 (314 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1510
Search term: tplE

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 18290
Genomic Position 1640979
Position in ORF 1189(1710)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 6579
Genomic Position 1641200
Position in ORF 1410(1710)
Reference 14617778

Transposon Insertions in Orthologs

References

Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors.
Russell AB, LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD
Nature 2013 Apr;496(7446):508-12
PubMed ID: 23552891
The Pseudomonas aeruginosa Type VI Secretion PGAP1-like Effector Induces Host Autophagy by Activating Endoplasmic Reticulum Stress.
Jiang F, Wang X, Wang B, Chen L, Zhao Z, Waterfield NR, Yang G, Jin Q
Cell Rep 2016 Aug;16(6):1502-9
PubMed ID: 27477276