Pseudomonas aeruginosa PAO1, PA1719 (pscF)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA1719
Name	pscF
Replicon	chromosome
Genomic location	1862764 - 1863021 (+ strand)
Transposon Mutants	1 transposon mutants in PAO1

Cross-References

RefSeq	NP_250410.1
GI	15596916
Affymetrix	PA1719_pscF_at
DNASU	PaCD00006779
Entrez	879634
GenBank	AAG05108.1
INSDC	AAG05108.1
NCBI Locus Tag	PA1719
protein_id(GenBank)	gb|AAG05108.1|AE004598_7|gnl|PseudoCAP|PA1719
TIGR	NTL03PA01720
UniParc	UPI00000D4227
UniProtKB Acc	P95434
UniProtKB ID	PSCF_PSEAE
UniRef100	UniRef100_P95434
UniRef50	UniRef50_P95434
UniRef90	UniRef90_P95434

Product

Feature Type	CDS
Coding Frame	1
Product Name	type III export protein PscF Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7)	0.22
Kyte-Doolittle Hydrophobicity Value	-0.344
Molecular Weight (kDa)	9.3
Isoelectric Point (pI)	7.54

Subcellular localization

Individual Mappings	Localization Confidence PMID Extracellular Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
2UWJ	CHAPERONE	03/22/07	Structure of the heterotrimeric complex which regulates type III secretion needle formation	PSEUDOMONAS AERUGINOSA	2	X-RAY DIFFRACTION	100.0

Virulence Evidence

Source 1
Database	PseudoCAP
Category	Type III Secretion System
Evidence	ECO:0000315 mutant phenotype evidence used in manual assertion
Host Organism	Mus musculus
Notes	PscF has attenuated cytotoxicity in J774.2 murine macrophage-like cells
Reference	16239085
Source 2
Database	VFDB : VF0083
Category	T3SS
Evidence
Host Organism
Notes
Reference	11372029

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 5 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003670 (309 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA1719 Search term: pscF Search term: type III export protein PscF

Human Homologs

References

Analysis of virC, an operon involved in the secretion of Yop proteins by Yersinia enterocolitica. Michiels T, Vanooteghem JC, Lambert de Rouvroit C, China B, Gustin A, Boudry P, Cornelis GR J Bacteriol 1991 Aug;173(16):4994-5009 PubMed ID: 1860816

Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway. Yahr TL, Goranson J, Frank DW Mol Microbiol 1996 Dec;22(5):991-1003 PubMed ID: 8971719

PscF is a major component of the Pseudomonas aeruginosa type III secretion needle. Pastor A, Chabert J, Louwagie M, Garin J, Attree I FEMS Microbiol Lett 2005 Dec 1;253(1):95-101 PubMed ID: 16239085

Cross talk between type III secretion and flagellar assembly systems in Pseudomonas aeruginosa. Soscia C, Hachani A, Bernadac A, Filloux A, Bleves S J Bacteriol 2007 Apr;189(8):3124-32 PubMed ID: 17307856