Pseudomonas aeruginosa PAO1, PA1757 (thrH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1757
Name
thrH
Replicon chromosome
Genomic location 1896630 - 1897247 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250448.1
GI 15596954
Affymetrix PA1757_thrH_at
Entrez 877969
GenBank AAG05146.1
INSDC AAG05146.1
NCBI Locus Tag PA1757
protein_id(GenBank) gb|AAG05146.1|AE004601_11|gnl|PseudoCAP|PA1757
TIGR NTL03PA01758
UniParc UPI00000C5480
UniProtKB Acc Q9I2Y2
UniProtKB ID THRH_PSEAE
UniRef100 UniRef100_Q9I2Y2
UniRef50 UniRef50_Q9I2Y2
UniRef90 UniRef90_Q9I2Y2

Product

Feature Type CDS
Coding Frame 1
Product Name
homoserine kinase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.86
Kyte-Doolittle Hydrophobicity Value -0.091
Molecular Weight (kDa) 23.5
Isoelectric Point (pI) 4.98

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1RKV TRANSFERASE 11/23/03 Structure of Phosphate complex of ThrH from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0
1RKU TRANSFERASE 11/23/03 Crystal Structure of ThrH gene product of Pseudomonas Aeruginosa Pseudomonas aeruginosa 1.47 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 89 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003636 (458 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1757
Search term: thrH
Search term: homoserine kinase

Human Homologs

References

ThrH, a homoserine kinase isozyme with in vivo phosphoserine phosphatase activity in Pseudomonas aeruginosa.
Patte JC, Clepet C, Bally M, Borne F, Méjean V, Foglino M
Microbiology (Reading) 1999 Apr;145 ( Pt 4):845-853
PubMed ID: 10220164