Pseudomonas aeruginosa PAO1, PA1794 (glnS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1794
Name
glnS
Replicon chromosome
Genomic location 1943067 - 1944737 (+ strand)

Cross-References

RefSeq NP_250485.1
GI 15596991
Affymetrix PA1794_glnS_at
Entrez 878828
GenBank AAG05183.1
INSDC AAG05183.1
NCBI Locus Tag PA1794
protein_id(GenBank) gb|AAG05183.1|AE004605_5|gnl|PseudoCAP|PA1794
TIGR NTL03PA01795
UniParc UPI0000136622
UniProtKB Acc Q9I2U8
UniProtKB ID SYQ_PSEAE
UniRef100 UniRef100_Q9I2U8
UniRef50 UniRef50_Q9I2U8
UniRef90 UniRef90_Q9I2U8

Product

Feature Type CDS
Coding Frame 1
Product Name
glutaminyl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms glutamine-tRNA ligase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -12.45
Kyte-Doolittle Hydrophobicity Value -0.554
Molecular Weight (kDa) 62.9
Isoelectric Point (pI) 5.64

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5BNZ LIGASE 05/26/15 Crystal structure of Glutamine-tRNA ligase /Glutaminyl-tRNA synthetase (GlnRS) from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 1.9 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 620 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003604 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1794
Search term: glnS

Human Homologs

Ensembl 110, assembly GRCh38.p14
glutaminyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:9751]
E-value: 2.1e-131
Percent Identity: 47.8
Ensembl 110, assembly GRCh38.p14
glutaminyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:9751]
E-value: 2.1e-131
Percent Identity: 47.8
Ensembl 110, assembly GRCh38.p14
glutaminyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:9751]
E-value: 2.1e-131
Percent Identity: 47.8

References

Structural basis of anticodon loop recognition by glutaminyl-tRNA synthetase.
Rould MA, Perona JJ, Steitz TA
Nature 1991 Jul 18;352(6332):213-8
PubMed ID: 1857417
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994