Pseudomonas aeruginosa PAO1, PA1801 (clpP)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA1801
Name	clpP
Replicon	chromosome
Genomic location	1954069 - 1954710 (+ strand)
Transposon Mutants	1 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_250492.1
GI	15596998
Affymetrix	PA1801_clpP_at
DNASU	PaCD00006820
Entrez	878359
GenBank	AAG05190.1
INSDC	AAG05190.1
NCBI Locus Tag	PA1801
protein_id(GenBank)	gb|AAG05190.1|AE004606_4|gnl|PseudoCAP|PA1801
TIGR	NTL03PA01802
UniParc	UPI0000127AE8
UniProtKB Acc	Q9I2U1
UniProtKB ID	CLPP1_PSEAE
UniRef100	UniRef100_Q9I2U1
UniRef50	UniRef50_Q9I2U1
UniRef90	UniRef90_Q9I2U1

Product

Feature Type	CDS
Coding Frame	1
Product Name	ClpP Product Name Confidence: Class 1
Synonyms	protease ti
Evidence for Translation	Detected by MALDI-TOF/TOF (PMID:19333994). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-2.42
Kyte-Doolittle Hydrophobicity Value	-0.017
Molecular Weight (kDa)	23.5
Isoelectric Point (pI)	6.39

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7M1M	HYDROLASE	03/13/21	Crystal structure of Pseudomonas aeruginosa ClpP1	Pseudomonas aeruginosa	2.6	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 582 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003596 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA1801 Search term: clpP Search term: ClpP

Human Homologs

Ensembl 110, assembly GRCh38.p14

ENSP00000245816 caseinolytic mitochondrial matrix peptidase proteolytic subunit [Source:HGNC Symbol;Acc:HGNC:2084] E-value: 4.7e-60 Percent Identity: 59.0

References

The ClpX heat-shock protein of Escherichia coli, the ATP-dependent substrate specificity component of the ClpP-ClpX protease, is a novel molecular chaperone. Wawrzynow A, Wojtkowiak D, Marszalek J, Banecki B, Jonsen M, Graves B, Georgopoulos C, Zylicz M EMBO J 1995 May 1;14(9):1867-77 PubMed ID: 7743994

Molecular symmetry of the ClpP component of the ATP-dependent Clp protease, an Escherichia coli homolog of 20 S proteasome. Shin DH, Lee CS, Chung CH, Suh SW J Mol Biol 1996 Sep 20;262(2):71-6 PubMed ID: 8831780

Enzymatic and structural similarities between the Escherichia coli ATP-dependent proteases, ClpXP and ClpAP. Grimaud R, Kessel M, Beuron F, Steven AC, Maurizi MR J Biol Chem 1998 May 15;273(20):12476-81 PubMed ID: 9575205

ClpXP proteases positively regulate alginate overexpression and mucoid conversion in Pseudomonas aeruginosa. Qiu D, Eisinger VM, Head NE, Pier GB, Yu HD Microbiology (Reading) 2008 Jul;154(Pt 7):2119-2130 PubMed ID: 18599839

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Role of intracellular proteases in the antibiotic resistance, motility, and biofilm formation of Pseudomonas aeruginosa. Fernández L, Breidenstein EB, Song D, Hancock RE Antimicrob Agents Chemother 2012 Feb;56(2):1128-32 PubMed ID: 22123702