Pseudomonas aeruginosa PAO1, PA1803 (lon)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1803
Name
lon
Synonym: muc
Synonym: lopA
Synonym: capR
Synonym: deg
Replicon chromosome
Genomic location 1956227 - 1958623 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
Comment"Lon protease is essential for biofilm formation

Cross-References

RefSeq NP_250494.1
GI 15597000
Affymetrix PA1803_lon_at
Entrez 877926
GenBank AAG05192.1
INSDC AAG05192.1
NCBI Locus Tag PA1803
protein_id(GenBank) gb|AAG05192.1|AE004606_6|gnl|PseudoCAP|PA1803
TIGR NTL03PA01804
UniParc UPI00000C54A4
UniProtKB Acc Q9I2T9
UniProtKB ID Q9I2T9_PSEAE
UniRef100 UniRef100_Q9I2T9
UniRef50 UniRef50_P37945
UniRef90 UniRef90_Q9I2T9

Product

Feature Type CDS
Coding Frame 1
Product Name
Lon protease
Product Name Confidence: Class 1
Synonyms CapR protein
Atp-dependent protease La
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.95
Kyte-Doolittle Hydrophobicity Value -0.279
Molecular Weight (kDa) 88.6
Isoelectric Point (pI) 6.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category Lon protease
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Dictyostelium discoideum
Notes PAO1 lon mutant exhbited a twofold reduction in the number of amoeba cells required to form a plaque
Reference 23145092

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 572 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003594 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1803
Search term: lon
Search term: Lon protease

Human Homologs

Ensembl 110, assembly GRCh38.p14
lon peptidase 2, peroxisomal [Source:HGNC Symbol;Acc:HGNC:20598]
E-value: 6.4e-142
Percent Identity: 37.6

References

Regulatory region of the heat shock-inducible capR (lon) gene: DNA and protein sequences.
Gayda RC, Stephens PE, Hewick R, Schoemaker JM, Dreyer WJ, Markovitz A
J Bacteriol 1985 Apr;162(1):271-5
PubMed ID: 2984174
Controlled high-level expression of the lon gene of Escherichia coli allows overproduction of Lon protease.
Thomas CD, Modha J, Razzaq TM, Cullis PM, Rivett AJ
Gene 1993 Dec 22;136(1-2):237-42
PubMed ID: 8294008
Redundant in vivo proteolytic activities of Escherichia coli Lon and the ClpYQ (HslUV) protease.
Wu WF, Zhou Y, Gottesman S
J Bacteriol 1999 Jun;181(12):3681-7
PubMed ID: 10368141
Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli.
Kneidinger B, Marolda C, Graninger M, Zamyatina A, McArthur F, Kosma P, Valvano MA, Messner P
J Bacteriol 2002 Jan;184(2):363-9
PubMed ID: 11751812
Negative regulation of quorum-sensing systems in Pseudomonas aeruginosa by ATP-dependent Lon protease.
Takaya A, Tabuchi F, Tsuchiya H, Isogai E, Yamamoto T
J Bacteriol 2008 Jun;190(12):4181-8
PubMed ID: 18408026
Role of lon, an ATP-dependent protease homolog, in resistance of Pseudomonas aeruginosa to ciprofloxacin.
Brazas MD, Breidenstein EB, Overhage J, Hancock RE
Antimicrob Agents Chemother 2007 Dec;51(12):4276-83
PubMed ID: 17893152
Complex ciprofloxacin resistome revealed by screening a Pseudomonas aeruginosa mutant library for altered susceptibility.
Breidenstein EB, Khaira BK, Wiegand I, Overhage J, Hancock RE
Antimicrob Agents Chemother 2008 Dec;52(12):4486-91
PubMed ID: 18824609
The Lon protease is essential for full virulence in Pseudomonas aeruginosa.
Breidenstein EB, Janot L, Strehmel J, Fernandez L, Taylor PK, Kukavica-Ibrulj I, Gellatly SL, Levesque RC, Overhage J, Hancock RE
PLoS One 2012;7(11):e49123
PubMed ID: 23145092