Pseudomonas aeruginosa PAO1, PA1807 (nppD)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA1807
Name	nppD Synonym: oppE
Replicon	chromosome
Genomic location	1962046 - 1963656 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_250498.1
GI	15597004
Affymetrix	PA1807_at
Entrez	878351
GenBank	AAG05196.1
INSDC	AAG05196.1
NCBI Locus Tag	PA1807
protein_id(GenBank)	gb|AAG05196.1|AE004607_2|gnl|PseudoCAP|PA1807
TIGR	NTL03PA01808
UniParc	UPI00000C54A6
UniProtKB Acc	Q9I2T7
UniProtKB ID	Q9I2T7_PSEAE
UniRef100	UniRef100_A0A072ZHQ2
UniRef50	UniRef50_G8SAH9
UniRef90	UniRef90_B7VB70

Product

Feature Type	CDS
Coding Frame	1
Product Name	NppD Product Name Confidence: Class 1
Synonyms	probable ATP-binding component of ABC transporter
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected using LC-MS/MS (59.07 kDa, PMID:21751344)
Charge (pH 7)	2.99
Kyte-Doolittle Hydrophobicity Value	-0.080
Molecular Weight (kDa)	59.1
Isoelectric Point (pI)	7.52

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 2 Cytoplasmic Membrane Class 3 20472543 Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003590 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA1807 Search term: nppD Search term: NppD

Human Homologs

Ensembl 110, assembly GRCh38.p14	ENSP00000499543 ATP binding cassette subfamily B member 7 [Source:HGNC Symbol;Acc:HGNC:48] E-value: 6.4e-21 Percent Identity: 31.2
Ensembl 110, assembly GRCh38.p14	ENSP00000343849 ATP binding cassette subfamily B member 7 [Source:HGNC Symbol;Acc:HGNC:48] E-value: 6.4e-21 Percent Identity: 31.2
Ensembl 110, assembly GRCh38.p14	ENSP00000496526 ATP binding cassette subfamily B member 7 [Source:HGNC Symbol;Acc:HGNC:48] E-value: 6.4e-21 Percent Identity: 31.2
Ensembl 110, assembly GRCh38.p14	ENSP00000253577 ATP binding cassette subfamily B member 7 [Source:HGNC Symbol;Acc:HGNC:48] E-value: 6.4e-21 Percent Identity: 31.2
Ensembl 110, assembly GRCh38.p14	ENSP00000479985 ATP binding cassette subfamily B member 7 [Source:HGNC Symbol;Acc:HGNC:48] E-value: 6.4e-21 Percent Identity: 31.2
Ensembl 110, assembly GRCh38.p14	ENSP00000436586 ATP binding cassette subfamily B member 7 [Source:HGNC Symbol;Acc:HGNC:48] E-value: 6.4e-21 Percent Identity: 31.2
Ensembl 110, assembly GRCh38.p14	ENSP00000362492 ATP binding cassette subfamily B member 7 [Source:HGNC Symbol;Acc:HGNC:48] E-value: 6.4e-21 Percent Identity: 31.2

References

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344

The Pseudomonas aeruginosa PA14 ABC Transporter NppA1A2BCD Is Required for Uptake of Peptidyl Nucleoside Antibiotics. Pletzer D, Braun Y, Dubiley S, Lafon C, Köhler T, Page MGP, Mourez M, Severinov K, Weingart H J Bacteriol 2015 Jul;197(13):2217-2228 PubMed ID: 25917903