Pseudomonas aeruginosa PAO1, PA1809 (nppB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1809
Name
nppB
Synonym: oppC
Replicon chromosome
Genomic location 1964682 - 1965764 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_250500.1
GI 15597006
Affymetrix PA1809_at
Entrez 878361
GenBank AAG05198.1
INSDC AAG05198.1
NCBI Locus Tag PA1809
protein_id(GenBank) gb|AAG05198.1|AE004607_4|gnl|PseudoCAP|PA1809
TIGR NTL03PA01810
UniParc UPI00000C54A8
UniProtKB Acc Q9I2T5
UniProtKB ID Q9I2T5_PSEAE
UniRef100 UniRef100_S0IDM7
UniRef50 UniRef50_P0AFU1
UniRef90 UniRef90_A0A024HFR4

Product

Feature Type CDS
Coding Frame 1
Product Name
NppB
Product Name Confidence: Class 1
Synonyms probable permease of ABC transporter
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.04
Kyte-Doolittle Hydrophobicity Value 0.656
Molecular Weight (kDa) 39.6
Isoelectric Point (pI) 6.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 582 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003588 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1809
Search term: nppB
Search term: NppB

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.
Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T
DNA Res 1996 Dec 31;3(6):379-92
PubMed ID: 9097040
The Pseudomonas aeruginosa PA14 ABC Transporter NppA1A2BCD Is Required for Uptake of Peptidyl Nucleoside Antibiotics.
Pletzer D, Braun Y, Dubiley S, Lafon C, Köhler T, Page MGP, Mourez M, Severinov K, Weingart H
J Bacteriol 2015 Jul;197(13):2217-2228
PubMed ID: 25917903