Pseudomonas aeruginosa PAO1, PA1818 (cadA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1818
Name
cadA
Synonym: ldcA
Synonym: adi
Synonym: ldcC
Synonym: speC
Replicon chromosome
Genomic location 1974821 - 1977076 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentModeling evidence, since PAO1 grows on lysine. Also physiological evidence from Fothergill et al. BLAST of Lysine decarboxylase Burkholderia cenocepacia HI2424 vs. P. aeruginosa PAO1 genome gave E = 0 with PA1818. Orn and Arg functions are also in reconstruction.

Cross-References

RefSeq NP_250509.1
GI 15597015
Affymetrix PA1818_at
Entrez 878596
GenBank AAG05207.1
INSDC AAG05207.1
NCBI Locus Tag PA1818
protein_id(GenBank) gb|AAG05207.1|AE004608_6|gnl|PseudoCAP|PA1818
TIGR NTL03PA01819
UniParc UPI00000C54AF
UniProtKB Acc Q9I2S7
UniProtKB ID Q9I2S7_PSEAE
UniRef100 UniRef100_Q9I2S7
UniRef50 UniRef50_I7KDU0
UniRef90 UniRef90_Q9I2S7

Product

Feature Type CDS
Coding Frame 1
Product Name
lysine decarboxylase
Product Name Confidence: Class 2
Synonyms Orn/Arg/Lys decarboxylase LdcC
lysine-specific pyridoxal 5'-phosphate-dependent carboxylase, LdcA
lysine-specific pyridoxal 5’-phosphate-dependent carboxylase, LdcA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.13
Kyte-Doolittle Hydrophobicity Value -0.083
Molecular Weight (kDa) 82.8
Isoelectric Point (pI) 6.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 242 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003580 (491 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1818
Search term: cadA

References

Catabolism of L-lysine by Pseudomonas aeruginosa.
Fothergill JC, Guest JR
J. Gen. Microbiol. 1977 Mar;99(1):139-55
PubMed ID: 405455
CzcR-CzcS, a two-component system involved in heavy metal and carbapenem resistance in Pseudomonas aeruginosa.
Perron K, Caille O, Rossier C, Van Delden C, Dumas JL, Köhler T
J. Biol. Chem. 2004 Mar;279(10):8761-8
PubMed ID: 14679195
Genes and enzymes of lysine catabolism in Pseudomonas aeruginosa.
Rahman M, Clarke PH
J. Gen. Microbiol. 1980 Feb;116(2):357-69
PubMed ID: 6768834
L-lysine catabolism is controlled by L-arginine and ArgR in Pseudomonas aeruginosa PAO1.
Chou HT, Hegazy M, Lu CD
J. Bacteriol. 2010 Nov;192(22):5874-80
PubMed ID: 20833801
Multiple and interconnected pathways for L-lysine catabolism in Pseudomonas putida KT2440.
Revelles O, Espinosa-Urgel M, Fuhrer T, Sauer U, Ramos JL
J. Bacteriol. 2005 Nov;187(21):7500-10
PubMed ID: 16237033