Pseudomonas aeruginosa PAO1, PA1818 (cadA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1818
Name
cadA
Synonym: ldcA
Synonym: adi
Synonym: ldcC
Synonym: speC
Replicon chromosome
Genomic location 1974821 - 1977076 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_250509.1
GI 15597015
Affymetrix PA1818_at
Entrez 878596
GenBank AAG05207.1
INSDC AAG05207.1
NCBI Locus Tag PA1818
protein_id(GenBank) gb|AAG05207.1|AE004608_6|gnl|PseudoCAP|PA1818
TIGR NTL03PA01819
UniParc UPI00000C54AF
UniProtKB Acc Q9I2S7
UniProtKB ID Q9I2S7_PSEAE
UniRef100 UniRef100_Q9I2S7
UniRef50 UniRef50_I7KDU0
UniRef90 UniRef90_Q9I2S7

Product

Feature Type CDS
Coding Frame 1
Product Name
lysine decarboxylase
Product Name Confidence: Class 2
CommentModeling evidence, since PAO1 grows on lysine. Also physiological evidence from Fothergill et al. BLAST of Lysine decarboxylase Burkholderia cenocepacia HI2424 vs. P. aeruginosa PAO1 genome gave E = 0 with PA1818. Orn and Arg functions are also in reconstruction.
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.13
Kyte-Doolittle Hydrophobicity Value -0.083
Molecular Weight (kDa) 82.8
Isoelectric Point (pI) 6.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 242 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003580 (491 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1818
Search term: cadA

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 3295
Genomic Position 1975592
Position in ORF 772(2256)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 8541
Genomic Position 1976841
Position in ORF 2021(2256)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 2005
Genomic Position 1976981
Position in ORF 2161(2256)
Reference 14617778

Transposon Insertions in Orthologs

References

Catabolism of L-lysine by Pseudomonas aeruginosa.
Fothergill JC, Guest JR
J. Gen. Microbiol. 1977 Mar;99(1):139-55
PubMed ID: 405455
CzcR-CzcS, a two-component system involved in heavy metal and carbapenem resistance in Pseudomonas aeruginosa.
Perron K, Caille O, Rossier C, Van Delden C, Dumas JL, Köhler T
J. Biol. Chem. 2004 Mar;279(10):8761-8
PubMed ID: 14679195
Genes and enzymes of lysine catabolism in Pseudomonas aeruginosa.
Rahman M, Clarke PH
J. Gen. Microbiol. 1980 Feb;116(2):357-69
PubMed ID: 6768834
L-lysine catabolism is controlled by L-arginine and ArgR in Pseudomonas aeruginosa PAO1.
Chou HT, Hegazy M, Lu CD
J. Bacteriol. 2010 Nov;192(22):5874-80
PubMed ID: 20833801
Multiple and interconnected pathways for L-lysine catabolism in Pseudomonas putida KT2440.
Revelles O, Espinosa-Urgel M, Fuhrer T, Sauer U, Ramos JL
J. Bacteriol. 2005 Nov;187(21):7500-10
PubMed ID: 16237033