Pseudomonas aeruginosa PAO1, PA1998 (dchR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1998
Name
dchR
Synonym: dhcR
Replicon chromosome
Genomic location 2184475 - 2185395 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_250688.1
GI 15597194
Affymetrix PA1998_at
DNASU PaCD00006893
Entrez 879084
GenBank AAG05386.1
INSDC AAG05386.1
NCBI Locus Tag PA1998
protein_id(GenBank) gb|AAG05386.1|AE004626_5|gnl|PseudoCAP|PA1998
TIGR NTL03PA01998
UniParc UPI00000C554D
UniProtKB Acc Q9I2B1
UniProtKB ID Q9I2B1_PSEAE
UniRef100 UniRef100_Q9I2B1
UniRef50 UniRef50_Q9I2B1
UniRef90 UniRef90_Q9I2B1

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator, DchR
Product Name Confidence: Class 1
CommentDeletion of dhcR or transposon insertions into the coding sequence result in a strain unable to grow using L-carnitine as a sole carbon source. DhcR regulates the transcriptional induction of the adjacent dhcAB genes in response to an L-carnitine catabolite predicted to be 3-dehydrocarnitine.
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.13
Kyte-Doolittle Hydrophobicity Value 0.031
Molecular Weight (kDa) 33.9
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 547 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003437 (1087 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1998
Search term: dchR

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 34189
Genomic Position 2184985
Position in ORF 511(921)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 3
Genomic Position 2184888
Position in ORF 414(921)
Reference 14617778

Transposon Insertions in Orthologs

References

Identification of genes required for Pseudomonas aeruginosa carnitine catabolism.
Wargo MJ, Hogan DA
Microbiology (Reading, Engl.) 2009 Jul;155(Pt 7):2411-9
PubMed ID: 19406895
Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1.
Madhuri Indurthi S, Chou HT, Lu CD
Microbiology (Reading, Engl.) 2016 May;162(5):876-88
PubMed ID: 26967762