Pseudomonas aeruginosa PAO1, PA1999 (dchA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1999
Name
dchA
Synonym: pcaI
Synonym: dhcA
Synonym: atoD
Synonym: scoA
Replicon chromosome
Genomic location 2185527 - 2186225 (+ strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250689.1
GI 15597195
Affymetrix PA1999_at
DNASU PaCD00006894
Entrez 878889
GenBank AAG05387.1
INSDC AAG05387.1
NCBI Locus Tag PA1999
protein_id(GenBank) gb|AAG05387.1|AE004626_6|gnl|PseudoCAP|PA1999
TIGR NTL03PA01999
UniParc UPI00000C554E
UniProtKB Acc Q9I2B0
UniProtKB ID Q9I2B0_PSEAE
UniRef100 UniRef100_W0YSZ8
UniRef50 UniRef50_P42315
UniRef90 UniRef90_A0A024HH44

Product

Feature Type CDS
Coding Frame 1
Product Name
dehydrocarnitine CoA transferase, DchA
Product Name Confidence: Class 1
CommentDeletion of dhcA or transposon insertions into the coding sequence result in a strain unable to grow using L-carnitine as a sole carbon source. DhcA is predicted to form a complex with DhcB which functions to deacetylate 3-dehydrocarnitine generating acetyl-CoA and glycine betaine CoA. Deletion of the dhcAB operon results in complete loss of carnitine-dependent CoA utilization from cytoplasmic extracts compared to cytoplasm from wild-type cells.; Transcription of the dhcAB operon is regulated by the transcription factor DhcR.
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.09
Kyte-Doolittle Hydrophobicity Value -0.127
Molecular Weight (kDa) 25.2
Isoelectric Point (pI) 5.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 292 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003436 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1999
Search term: dchA

Human Homologs

Ensembl 86, assembly GRCh38.p7
3-oxoacid CoA-transferase 1 [Source:HGNC Symbol;Acc:HGNC:8527]
E-value: 3.5e-75
Percent Identity: 60.1

Transposon Insertions in PAO1

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_38660
Mutant ID PA14NR: 30401
Genomic Position 3448639
Position in ORF 677(699)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_38660
Mutant ID PA14NR: 53819
Genomic Position 3448424
Position in ORF 462(699)
Reference 16477005

References

Succinyl CoA: 3-oxoacid CoA transferase (SCOT): human cDNA cloning, human chromosomal mapping to 5p13, and mutation detection in a SCOT-deficient patient.
Kassovska-Bratinova S, Fukao T, Song XQ, Duncan AM, Chen HS, Robert MF, Pérez-Cerdá C, Ugarte M, Chartrand C, Vobecky S, Kondo N, Mitchell GA
Am. J. Hum. Genet. 1996 Sep;59(3):519-28
PubMed ID: 8751852
Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1.
Madhuri Indurthi S, Chou HT, Lu CD
Microbiology (Reading, Engl.) 2016 May;162(5):876-88
PubMed ID: 26967762