Pseudomonas aeruginosa PAO1, PA2000 (dchB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2000
Name
dchB
Synonym: dhcB
Synonym: atoA
Synonym: scoB
Replicon chromosome
Genomic location 2186260 - 2186916 (+ strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250690.1
GI 15597196
Affymetrix PA2000_at
DNASU PaCD00006895
Entrez 878555
GenBank AAG05388.1
INSDC AAG05388.1
NCBI Locus Tag PA2000
protein_id(GenBank) gb|AAG05388.1|AE004626_7|gnl|PseudoCAP|PA2000
TIGR NTL03PA02000
UniParc UPI00000C554F
UniProtKB Acc Q9I2A9
UniProtKB ID Q9I2A9_PSEAE
UniRef100 UniRef100_Q9I2A9
UniRef50 UniRef50_Q9I2A9
UniRef90 UniRef90_Q9I2A9

Product

Feature Type CDS
Coding Frame 1
Product Name
dehydrocarnitine CoA transferase, DchB
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.37
Kyte-Doolittle Hydrophobicity Value 0.243
Molecular Weight (kDa) 23.2
Isoelectric Point (pI) 4.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 271 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003435 (563 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2000
Search term: dchB

Human Homologs

Ensembl 86, assembly GRCh38.p7
3-oxoacid CoA-transferase 1 [Source:HGNC Symbol;Acc:HGNC:8527]
E-value: 1.9e-54
Percent Identity: 51.7

Transposon Insertions in PAO1

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_38640
Mutant ID PA14NR: 34027
Genomic Position 3447400
Position in ORF 129(657)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_38640
Mutant ID PA14NR: 40012
Genomic Position 3447868
Position in ORF 597(657)
Reference 16477005

References

Identification of glutamate 344 as the catalytic residue in the active site of pig heart CoA transferase.
Rochet JC, Bridger WA.
Protein Sci. 1994 Jun;3(6):975-8
PubMed ID: 7915164
Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1.
Madhuri Indurthi S(1), Chou HT(1), Lu CD(2).
Microbiology. 2016 May;162(5):876-88.
PubMed ID: 26967762