Pseudomonas aeruginosa PAO1, PA2003 (bdhA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2003
Name
bdhA
Replicon chromosome
Genomic location 2190084 - 2190854 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 5 transposon mutants in orthologs

Cross-References

RefSeq NP_250693.1
GI 15597199
Affymetrix PA2003_bdhA_at
Entrez 879996
GenBank AAG05391.1
INSDC AAG05391.1
NCBI Locus Tag PA2003
protein_id(GenBank) gb|AAG05391.1|AE004626_10|gnl|PseudoCAP|PA2003
TIGR NTL03PA02003
UniParc UPI00000C5552
UniProtKB Acc Q9I2A6
UniProtKB ID Q9I2A6_PSEAE
UniRef100 UniRef100_Q9I2A6
UniRef50 UniRef50_D0VWQ0
UniRef90 UniRef90_V6AFQ6

Product

Feature Type CDS
Coding Frame 1
Product Name
3-hydroxybutyrate dehydrogenase
Product Name Confidence: Class 1
Synonyms D-beta-hydroxybutyrate dehydrogenase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.65
Kyte-Doolittle Hydrophobicity Value 0.211
Molecular Weight (kDa) 26.7
Isoelectric Point (pI) 5.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 651 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000180 (2633 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2003
Search term: bdhA

Human Homologs

Ensembl 110, assembly GRCh38.p14
retinol dehydrogenase 8 [Source:HGNC Symbol;Acc:HGNC:14423]
E-value: 6.4e-11
Percent Identity: 28.4
Ensembl 110, assembly GRCh38.p14
retinol dehydrogenase 8 [Source:HGNC Symbol;Acc:HGNC:14423]
E-value: 6.4e-11
Percent Identity: 28.4

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Poly-3-hydroxybutyrate degradation in Rhizobium (Sinorhizobium) meliloti: isolation and characterization of a gene encoding 3-hydroxybutyrate dehydrogenase.
Aneja P, Charles TC
J Bacteriol 1999 Feb;181(3):849-57
PubMed ID: 9922248
Structural and catalytic properties of the D-3-hydroxybutyrate dehydrogenase from Pseudomonas aeruginosa.
Mountassif D, Andreoletti P, Cherkaoui-Malki M, Latruffe N, El Kebbaj MS
Curr Microbiol 2010 Jul;61(1):7-12
PubMed ID: 20052585