Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA2011
|
Name |
liuE
Synonym: gnyL Synonym: mvaB |
Replicon | chromosome |
Genomic location | 2199762 - 2200664 (- strand) |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_250701.1 |
GI | 15597207 |
Affymetrix | PA2011_at |
DNASU | PaCD00006898 |
Entrez | 878857 |
GenBank | AAG05399.1 |
INSDC | AAG05399.1 |
NCBI Locus Tag | PA2011 |
protein_id(GenBank) | gb|AAG05399.1|AE004627_7|gnl|PseudoCAP|PA2011 |
TIGR | NTL03PA02011 |
UniParc | UPI00000D429D |
UniProtKB Acc | Q9I2A0 |
UniProtKB ID | LIUE_PSEAE |
UniRef100 | UniRef100_Q9I2A0 |
UniRef50 | UniRef50_Q9I2A0 |
UniRef90 | UniRef90_Q9I2A0 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
3-hydroxy-3-methylglutaryl-CoA lyase
|
Synonyms |
Hydroxymethylglutaryl-coenzymeA lyase, GnyH HMG-CoA lyase HMG lyase, MvaB 3-hydroxy-gamma-carboxygeranoyl-CoA lyase, GnyL |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -2.98 |
Kyte-Doolittle Hydrophobicity Value | 0.059 |
Molecular Weight (kDa) | 31.8 |
Isoelectric Point (pI) | 6.04 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
2FTP | LYASE | 01/24/06 | Crystal Structure of hydroxymethylglutaryl-CoA lyase from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2.4 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 338 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG003428 (534 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA2011
Search term: liuE
Search term: 3-hydroxy-3-methylglutaryl-CoA lyase
|
Ensembl
110, assembly
GRCh38.p14
|
3-hydroxy-3-methylglutaryl-CoA lyase [Source:HGNC Symbol;Acc:HGNC:5005]
E-value:
1.1e-97
Percent Identity:
57.7
|
The gnyRDBHAL cluster is involved in acyclic isoprenoid degradation in Pseudomonas aeruginosa.
Díaz-Pérez AL, Zavala-Hernández AN, Cervantes C, Campos-García J
Appl Environ Microbiol 2004 Sep;70(9):5102-10
PubMed ID: 15345388
|
The atu and liu clusters are involved in the catabolic pathways for acyclic monoterpenes and leucine in Pseudomonas aeruginosa.
Aguilar JA, Zavala AN, Díaz-Pérez C, Cervantes C, Díaz-Pérez AL, Campos-García J
Appl Environ Microbiol 2006 Mar;72(3):2070-9
PubMed ID: 16517656
|
Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved in leucine/isovalerate utilization (Liu) and acyclic terpene utilization (Atu), and are encoded by liuB/liuD and atuC/atuF, in Pseudomonas aeruginosa.
Höschle B, Gnau V, Jendrossek D
Microbiology (Reading) 2005 Nov;151(Pt 11):3649-3656
PubMed ID: 16272386
|
Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine/isovalerate in Pseudomonas aeruginosa.
Förster-Fromme K, Höschle B, Mack C, Bott M, Armbruster W, Jendrossek D
Appl Environ Microbiol 2006 Jul;72(7):4819-28
PubMed ID: 16820476
|
Nucleotide sequence and expression in Escherichia coli of the 3-hydroxy-3-methylglutaryl coenzyme A lyase gene of Pseudomonas mevalonii.
Anderson DH, Rodwell VW
J Bacteriol 1989 Dec;171(12):6468-72
PubMed ID: 2687236
|
3-Hydroxy-3-methylglutaryl coenzyme A lyase: affinity labeling of the Pseudomonas mevalonii enzyme and assignment of cysteine-237 to the active site.
Hruz PW, Narasimhan C, Miziorko HM
Biochemistry 1992 Jul 28;31(29):6842-7
PubMed ID: 1637819
|
The Pseudomonas aeruginosa liuE gene encodes the 3-hydroxy-3-methylglutaryl coenzyme A lyase, involved in leucine and acyclic terpene catabolism.
Chávez-Avilés M, Díaz-Pérez AL, Reyes-de la Cruz H, Campos-García J
FEMS Microbiol Lett 2009 Jul;296(1):117-23
PubMed ID: 19459965
|