Pseudomonas aeruginosa PAO1, PA2020 (mexZ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2020
Name
mexZ
Synonym: mexZ
Synonym: amrR
Replicon chromosome
Genomic location 2212677 - 2213309 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250710.1
GI 15597216
Affymetrix PA2020_at
Entrez 880025
GenBank AAG05408.1
INSDC AAG05408.1
NCBI Locus Tag PA2020
protein_id(GenBank) gb|AAG05408.1|AE004628_6|gnl|PseudoCAP|PA2020
TIGR NTL03PA02020
UniParc UPI00000D421F
UniProtKB Acc G3XCU9
UniProtKB ID G3XCU9_PSEAE
UniRef100 UniRef100_W0YRR0
UniRef50 UniRef50_Q2SVT2
UniRef90 UniRef90_W0YRR0

Product

Feature Type CDS
Coding Frame 1
Product Name
MexZ
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) 0.06
Kyte-Doolittle Hydrophobicity Value -0.350
Molecular Weight (kDa) 23.7
Isoelectric Point (pI) 7.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2WUI TRANSCRIPTION 10/05/09 Crystal Structure of MexZ, a key repressor responsible for antibiotic resistance in Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 2.9 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 249 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003420 (241 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2020
Search term: mexZ
Search term: MexZ

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 883
Genomic Position 2212860
Position in ORF 184(633)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 50972
Genomic Position 2212930
Position in ORF 254(633)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_38380
Mutant ID PA14NR: 41094
Genomic Position 3421485
Position in ORF 597(633)
Reference 16477005

References

MexZ-mediated regulation of mexXY multidrug efflux pump expression in Pseudomonas aeruginosa by binding on the mexZ-mexX intergenic DNA.
Matsuo Y, Eda S, Gotoh N, Yoshihara E, Nakae T
FEMS Microbiol. Lett. 2004 Sep;238(1):23-8
PubMed ID: 15336398
Aminoglycoside resistance of Pseudomonas aeruginosa in cystic fibrosis results from convergent evolution in the mexZ gene.
Prickett MH, Hauser AR, McColley SA, Cullina J, Potter E, Powers C, Jain M
Thorax 2017 Jan;72(1):40-47
PubMed ID: 27325751