Pseudomonas aeruginosa PAO1, PA2020 (mexZ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2020
Name
mexZ
Synonym: mexZ
Synonym: amrR
Replicon chromosome
Genomic location 2212677 - 2213309 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250710.1
GI 15597216
Affymetrix PA2020_at
Entrez 880025
GenBank AAG05408.1
INSDC AAG05408.1
NCBI Locus Tag PA2020
protein_id(GenBank) gb|AAG05408.1|AE004628_6|gnl|PseudoCAP|PA2020
TIGR NTL03PA02020
UniParc UPI00000D421F
UniProtKB Acc G3XCU9
UniProtKB ID G3XCU9_PSEAE
UniRef100 UniRef100_W0YRR0
UniRef50 UniRef50_Q2SVT2
UniRef90 UniRef90_W0YRR0

Product

Feature Type CDS
Coding Frame 1
Product Name
MexZ
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) 0.06
Kyte-Doolittle Hydrophobicity Value -0.350
Molecular Weight (kDa) 23.7
Isoelectric Point (pI) 7.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2WUI TRANSCRIPTION 10/05/09 Crystal Structure of MexZ, a key repressor responsible for antibiotic resistance in Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 2.9 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 249 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003420 (244 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2020
Search term: mexZ
Search term: MexZ

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 883
Genomic Position 2212860
Position in ORF 184(633)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 50972
Genomic Position 2212930
Position in ORF 254(633)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_38380
Mutant ID PA14NR: 41094
Genomic Position 3421485
Position in ORF 597(633)
Reference 16477005

References

MexZ-mediated regulation of mexXY multidrug efflux pump expression in Pseudomonas aeruginosa by binding on the mexZ-mexX intergenic DNA.
Matsuo Y, Eda S, Gotoh N, Yoshihara E, Nakae T.
FEMS Microbiol Lett. 2004 Sep 1;238(1):23-8.
PubMed ID: 15336398
Aminoglycoside resistance of Pseudomonas aeruginosa in cystic fibrosis results from convergent evolution in the mexZ gene.
Prickett MH(1), Hauser AR(1), McColley SA(2), Cullina J(3), Potter E(3), Powers C(3), Jain M(1).
Thorax. 2016 Jun 20. pii: thoraxjnl-2015-208027.
PubMed ID: 27325751