Pseudomonas aeruginosa PAO1, PA2058 (sppA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2058
Name
sppA
Replicon chromosome
Genomic location 2253819 - 2255627 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_250748.1
GI 15597254
Affymetrix PA2058_at
Entrez 878515
GenBank AAG05446.1
INSDC AAG05446.1
NCBI Locus Tag PA2058
protein_id(GenBank) gb|AAG05446.1|AE004632_5|gnl|PseudoCAP|PA2058
TIGR NTL03PA02058
UniParc UPI00000C557B
UniProtKB Acc Q9I257
UniProtKB ID Q9I257_PSEAE
UniRef100 UniRef100_A0A087L8R9
UniRef50 UniRef50_A0A0D0RCL5
UniRef90 UniRef90_W0YQ03

Product

Feature Type CDS
Coding Frame 1
Product Name
ABC transporter substrate-binding protein, SppA
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.89
Kyte-Doolittle Hydrophobicity Value -0.511
Molecular Weight (kDa) 69.2
Isoelectric Point (pI) 8.70

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 339 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003391 (845 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2058
Search term: sppA

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 37505
Genomic Position 2255515
Position in ORF 1697(1809)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 55079
Genomic Position 2255373
Position in ORF 1555(1809)
Reference 14617778

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Swarming motility is modulated by expression of the putative xenosiderophore transporter SppR-SppABCD in Pseudomonas aeruginosa PA14.
Pletzer D, Braun Y, Weingart H
Antonie Van Leeuwenhoek 2016 Jun;109(6):737-53
PubMed ID: 26995781