Pseudomonas aeruginosa PAO1, PA2081 (kynB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2081
Name
kynB
Replicon chromosome
Genomic location 2290339 - 2290980 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentKynB is involved in the aerobic degradation of L-tryptophan to anthranilate. This form of kynurenine formamidase (KFA) is nonhomologous to the eukarytic KFA characterized in mice and belongs to a different class of Zn-dependent hydrolases

Cross-References

RefSeq NP_250771.1
GI 15597277
Affymetrix PA2081_at
DNASU PaCD00006922
Entrez 878591
GenBank AAG05469.1
INSDC AAG05469.1
NCBI Locus Tag PA2081
protein_id(GenBank) gb|AAG05469.1|AE004635_7|gnl|PseudoCAP|PA2081
TIGR NTL03PA02081
UniParc UPI00000C5590
UniProtKB Acc Q9I234
UniProtKB ID KYNB_PSEAE
UniRef100 UniRef100_Q9I234
UniRef50 UniRef50_P0C8P4
UniRef90 UniRef90_Q9I234

Product

Feature Type CDS
Coding Frame 1
Product Name
kynurenine formamidase, KynB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.75
Kyte-Doolittle Hydrophobicity Value -0.060
Molecular Weight (kDa) 23.2
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4COB HYDROLASE 01/28/14 Crystal structure kynurenine formamidase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.37 X-RAY DIFFRACTION 99.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 193 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003374 (350 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2081
Search term: kynB

Human Homologs

References

Aerobic tryptophan degradation pathway in bacteria: novel kynurenine formamidase.
Kurnasov O, Jablonski L, Polanuyer B, Dorrestein P, Begley T, Osterman A
FEMS Microbiol Lett 2003 Oct 24;227(2):219-27
PubMed ID: 14592712