Pseudomonas aeruginosa PAO1, PA2200

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2200
Name
Replicon chromosome
Genomic location 2418723 - 2420318 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentOverexpression of PA2200 results in impaired TTSS-mediated cytotoxicity

Cross-References

RefSeq NP_250890.1
GI 15597396
Affymetrix PA2200_at
Entrez 881519
GenBank AAG05588.1
INSDC AAG05588.1
NCBI Locus Tag PA2200
protein_id(GenBank) gb|AAG05588.1|AE004646_9|gnl|PseudoCAP|PA2200
TIGR NTL03PA02200
UniParc UPI00000C5602
UniProtKB Acc Q9I1R7
UniProtKB ID Q9I1R7_PSEAE
UniRef100 UniRef100_Q9I1R7
UniRef50 UniRef50_W1MMD6
UniRef90 UniRef90_S0IK80

Product

Feature Type CDS
Coding Frame 1
Product Name
Cyclic-guanylate-specific phosphodiesterase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.81
Kyte-Doolittle Hydrophobicity Value -0.021
Molecular Weight (kDa) 58.3
Isoelectric Point (pI) 6.04

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 402 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003283 (345 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2200

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
Kulasakara H, Lee V, Brencic A, Liberati N, Urbach J, Miyata S, Lee DG, Neely AN, Hyodo M, Hayakawa Y, Ausubel FM, Lory S
Proc Natl Acad Sci U S A 2006 Feb 21;103(8):2839-44
PubMed ID: 16477007