Pseudomonas aeruginosa PAO1, PA2231 (pslA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2231
Name
pslA
Replicon chromosome
Genomic location 2453667 - 2455103 (+ strand)
CommentPA2231 and PA2232 play a role in biofilm initiation. psl cluster plays a role in cell-cell and/or cell-surface interaction in biofilm formation

Cross-References

RefSeq NP_250921.1
GI 15597427
Affymetrix PA2231_at
Entrez 879717
GenBank AAG05619.1
INSDC AAG05619.1
NCBI Locus Tag PA2231
protein_id(GenBank) gb|AAG05619.1|AE004649_10|gnl|PseudoCAP|PA2231
TIGR NTL03PA02231
UniParc UPI00000C561D
UniProtKB Acc Q9I1N8
UniProtKB ID Q9I1N8_PSEAE
UniRef100 UniRef100_Q9I1N8
UniRef50 UniRef50_Q4ZR89
UniRef90 UniRef90_A6V5N5

Product

Feature Type CDS
Coding Frame 1
Product Name
PslA
Product Name Confidence: Class 1
Synonyms PslA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 18.34
Kyte-Doolittle Hydrophobicity Value 0.195
Molecular Weight (kDa) 54.8
Isoelectric Point (pI) 10.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 517 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005117 (516 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2231
Search term: pslA
Search term: PslA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of cpsD, a gene essential for type III capsule expression in group B streptococci.
Rubens CE, Heggen LM, Haft RF, Wessels MR
Mol Microbiol 1993 May;8(5):843-55
PubMed ID: 8355611
Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid.
Stevenson G, Andrianopoulos K, Hobbs M, Reeves PR
J Bacteriol 1996 Aug;178(16):4885-93
PubMed ID: 8759852
Synthesis of the A-band polysaccharide sugar D-rhamnose requires Rmd and WbpW: identification of multiple AlgA homologues, WbpW and ORF488, in Pseudomonas aeruginosa.
Rocchetta HL, Pacan JC, Lam JS
Mol Microbiol 1998 Sep;29(6):1419-34
PubMed ID: 9781879
Assembly and development of the Pseudomonas aeruginosa biofilm matrix.
Ma L, Conover M, Lu H, Parsek MR, Bayles K, Wozniak DJ
PLoS Pathog 2009 Mar;5(3):e1000354
PubMed ID: 19325879
Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production.
Byrd MS, Sadovskaya I, Vinogradov E, Lu H, Sprinkle AB, Richardson SH, Ma L, Ralston B, Parsek MR, Anderson EM, Lam JS, Wozniak DJ
Mol Microbiol 2009 Aug;73(4):622-38
PubMed ID: 19659934