Pseudomonas aeruginosa PAO1, PA2234 (pslD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2234
Name
pslD
Replicon chromosome
Genomic location 2457510 - 2458280 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Commentpsl cluster plays a role in cell-cell and/or cell-surface interaction in biofilm formation.

Cross-References

RefSeq NP_250924.1
GI 15597430
Affymetrix PA2234_at
Entrez 878051
GenBank AAG05622.1
INSDC AAG05622.1
NCBI Locus Tag PA2234
protein_id(GenBank) gb|AAG05622.1|AE004649_13|gnl|PseudoCAP|PA2234
TIGR NTL03PA02234
UniParc UPI00000C5620
UniProtKB Acc Q9I1N5
UniProtKB ID Q9I1N5_PSEAE
UniRef100 UniRef100_W0YTP2
UniRef50 UniRef50_W0YTP2
UniRef90 UniRef90_W0YTP2

Product

Feature Type CDS
Coding Frame 1
Product Name
PslD
Product Name Confidence: Class 1
Synonyms PslD
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (27.91 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.44
Kyte-Doolittle Hydrophobicity Value -0.039
Molecular Weight (kDa) 27.9
Isoelectric Point (pI) 8.90

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Extracellular Class 1
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 164 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005119 (367 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2234
Search term: pslD
Search term: PslD

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
PslD is a secreted protein required for biofilm formation by Pseudomonas aeruginosa.
Campisano A, Schroeder C, Schemionek M, Overhage J, Rehm BH
Appl Environ Microbiol 2006 Apr;72(4):3066-8
PubMed ID: 16598021
Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production.
Byrd MS, Sadovskaya I, Vinogradov E, Lu H, Sprinkle AB, Richardson SH, Ma L, Ralston B, Parsek MR, Anderson EM, Lam JS, Wozniak DJ
Mol Microbiol 2009 Aug;73(4):622-38
PubMed ID: 19659934
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344