Pseudomonas aeruginosa PAO1, PA2254 (pvcA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2254
Name
pvcA
Replicon chromosome
Genomic location 2482045 - 2483031 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250944.1
GI 15597450
Affymetrix PA2254_pvcA_at
DNASU PaCD00006996
Entrez 878099
GenBank AAG05642.1
INSDC AAG05642.1
NCBI Locus Tag PA2254
protein_id(GenBank) gb|AAG05642.1|AE004651_4|gnl|PseudoCAP|PA2254
TIGR NTL03PA02254
UniParc UPI00000C5631
UniProtKB Acc Q9I1L5
UniProtKB ID Q9I1L5_PSEAE
UniRef100 UniRef100_Q9I1L5
UniRef50 UniRef50_Q9I1L5
UniRef90 UniRef90_Q9I1L5

Product

Feature Type CDS
Coding Frame 1
Product Name
paerucumarin biosynthesis protein PvcA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.89
Kyte-Doolittle Hydrophobicity Value -0.258
Molecular Weight (kDa) 37.1
Isoelectric Point (pI) 6.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3E59 TRANSFERASE 08/13/08 Crystal structure of the PvcA (PA2254) protein from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 21 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003235 (309 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2254
Search term: pvcA

Human Homologs

References

Novel pyoverdine biosynthesis gene(s) of Pseudomonas aeruginosa PAO.
Stintzi A, Cornelis P, Hohnadel D, Meyer JM, Dean C, Poole K, Kourambas S, Krishnapillai V
Microbiology (Reading) 1996 May;142 ( Pt 5):1181-1190
PubMed ID: 8704959
The pvc gene cluster of Pseudomonas aeruginosa: role in synthesis of the pyoverdine chromophore and regulation by PtxR and PvdS.
Stintzi A, Johnson Z, Stonehouse M, Ochsner U, Meyer JM, Vasil ML, Poole K
J Bacteriol 1999 Jul;181(13):4118-24
PubMed ID: 10383985
Paerucumarin, a new metabolite produced by the pvc gene cluster from Pseudomonas aeruginosa.
Clarke-Pearson MF, Brady SF
J Bacteriol 2008 Oct;190(20):6927-30
PubMed ID: 18689486
Three-dimensional structures of Pseudomonas aeruginosa PvcA and PvcB, two proteins involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin.
Drake EJ, Gulick AM
J Mol Biol 2008 Dec 5;384(1):193-205
PubMed ID: 18824174