Pseudomonas aeruginosa PAO1, PA2255 (pvcB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2255
Name
pvcB
Replicon chromosome
Genomic location 2483049 - 2483924 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250945.1
GI 15597451
Affymetrix PA2255_pvcB_at
Entrez 878457
GenBank AAG05643.1
INSDC AAG05643.1
NCBI Locus Tag PA2255
protein_id(GenBank) gb|AAG05643.1|AE004651_5|gnl|PseudoCAP|PA2255
TIGR NTL03PA02255
UniParc UPI00000C5632
UniProtKB Acc Q9I1L4
UniProtKB ID Q9I1L4_PSEAE
UniRef100 UniRef100_Q9I1L4
UniRef50 UniRef50_Q3JHW6
UniRef90 UniRef90_Q9I1L4

Product

Feature Type CDS
Coding Frame 1
Product Name
paerucumarin biosynthesis protein PvcB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -3.55
Kyte-Doolittle Hydrophobicity Value -0.477
Molecular Weight (kDa) 33.1
Isoelectric Point (pI) 6.64

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4YLM OXIDOREDUCTASE 03/05/15 Structure of PvcB, an Fe, alpha-ketoglutarate dependent oxygenase from an isonitrile synthetic pathway Pseudomonas aeruginosa (PAK) 2.05 X-RAY DIFFRACTION 100.0
3EAT OXIDOREDUCTASE 08/26/08 Crystal structure of the PvcB (PA2255) protein from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 22 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003234 (305 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2255
Search term: pvcB

Human Homologs

References

Novel pyoverdine biosynthesis gene(s) of Pseudomonas aeruginosa PAO.
Stintzi A, Cornelis P, Hohnadel D, Meyer JM, Dean C, Poole K, Kourambas S, Krishnapillai V
Microbiology (Reading) 1996 May;142 ( Pt 5):1181-1190
PubMed ID: 8704959
Three-dimensional structures of Pseudomonas aeruginosa PvcA and PvcB, two proteins involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin.
Drake EJ, Gulick AM
J Mol Biol 2008 Dec 5;384(1):193-205
PubMed ID: 18824174