Pseudomonas aeruginosa PAO1, PA2362 (dotU3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2362
Name
dotU3
Replicon chromosome
Genomic location 2612041 - 2612799 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251052.1
GI 15597558
Affymetrix PA2362_at
Entrez 882222
GenBank AAG05750.1
INSDC AAG05750.1
NCBI Locus Tag PA2362
protein_id(GenBank) gb|AAG05750.1|AE004662_5|gnl|PseudoCAP|PA2362
TIGR NTL03PA02362
UniParc UPI00000C568E
UniProtKB Acc Q9I1B7
UniProtKB ID Q9I1B7_PSEAE
UniRef100 UniRef100_Q9I1B7
UniRef50 UniRef50_G8QBI2
UniRef90 UniRef90_W0YU43

Product

Feature Type CDS
Coding Frame 1
Product Name
DotU3
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.17
Kyte-Doolittle Hydrophobicity Value -0.178
Molecular Weight (kDa) 28.1
Isoelectric Point (pI) 7.73

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category Hcp secretion island-3 encoded type VI secretion system (H3-T6SS)
Evidence ECO:0000317
genomic context evidence used in manual assertion
Host Organism Caenorhabditis elegans
Notes
Infection model Caenorhabditis elegans slow killing assay. H3-T6SS rendered inactive via clpV3 deletion.
Reference 24204589

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 28 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003158 (326 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2362
Search term: dotU3
Search term: DotU3

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.
Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordoñez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ
Science 2006 Jun 9;312(5779):1526-30
PubMed ID: 16763151
Divergent control of two type VI secretion systems by RpoN in Pseudomonas aeruginosa.
Sana TG, Soscia C, Tonglet CM, Garvis S, Bleves S
PLoS One 2013;8(10):e76030
PubMed ID: 24204589