Pseudomonas aeruginosa PAO1, PA2449 (gcsR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2449
Name
gcsR
Replicon chromosome
Genomic location 2750128 - 2751663 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_251139.1
GI 15597645
Affymetrix PA2449_at
Entrez 882923
GenBank AAG05837.1
INSDC AAG05837.1
NCBI Locus Tag PA2449
protein_id(GenBank) gb|AAG05837.1|AE004672_5|gnl|PseudoCAP|PA2449
TIGR NTL03PA02449
UniParc UPI00000C56DB
UniProtKB Acc Q9I133
UniProtKB ID Q9I133_PSEAE
UniRef100 UniRef100_W1MJR5
UniRef50 UniRef50_D7HWM2
UniRef90 UniRef90_A6V526

Product

Feature Type CDS
Coding Frame 1
Product Name
GcsR
Product Name Confidence: Class 1
CommentResponsible for transcriptional activation of the gcvH2-gcvP2-glyA2-sdaA-gcvT2 operon that encodes genes required for glycine metabolism
Evidence for Translation
Charge (pH 7) -6.78
Kyte-Doolittle Hydrophobicity Value -0.042
Molecular Weight (kDa) 55.7
Isoelectric Point (pI) 6.17

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 449 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003090 (529 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2449
Search term: gcsR
Search term: GcsR

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 45109
Genomic Position 2750273
Position in ORF 146(1536)
Reference 14617778

Transposon Insertions in Orthologs

References

Gene PA2449 is essential for glycine metabolism and pyocyanin biosynthesis in Pseudomonas aeruginosa PAO1.
Lundgren BR, Thornton W, Dornan MH, Villegas-Peñaranda LR, Boddy CN, Nomura CT
J. Bacteriol. 2013 May;195(9):2087-100
PubMed ID: 23457254
GcsR, a TyrR-Like Enhancer-Binding Protein, Regulates Expression of the Glycine Cleavage System in Pseudomonas aeruginosa PAO1.
Sarwar Z, Lundgren BR, Grassa MT, Wang MX, Gribble M, Moffat JF, Nomura CT
mSphere ;1(2):
PubMed ID: 27303730