Pseudomonas aeruginosa PAO1, PA2449 (gcsR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2449
Name
gcsR
Replicon chromosome
Genomic location 2750128 - 2751663 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251139.1
GI 15597645
Affymetrix PA2449_at
Entrez 882923
GenBank AAG05837.1
INSDC AAG05837.1
NCBI Locus Tag PA2449
protein_id(GenBank) gb|AAG05837.1|AE004672_5|gnl|PseudoCAP|PA2449
TIGR NTL03PA02449
UniParc UPI00000C56DB
UniProtKB Acc Q9I133
UniProtKB ID Q9I133_PSEAE
UniRef100 UniRef100_W1MJR5
UniRef50 UniRef50_D7HWM2
UniRef90 UniRef90_A6V526

Product

Feature Type CDS
Coding Frame 1
Product Name
GcsR
Product Name Confidence: Class 1
Synonyms transcriptional regulator
CommentResponsible for transcriptional activation of the gcvH2-gcvP2-glyA2-sdaA-gcvT2 operon that encodes genes required for glycine metabolism
Evidence for Translation
Charge (pH 7) -6.78
Kyte-Doolittle Hydrophobicity Value -0.042
Molecular Weight (kDa) 55.7
Isoelectric Point (pI) 6.17

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 449 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003090 (529 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2449
Search term: gcsR
Search term: GcsR

References

Gene PA2449 is essential for glycine metabolism and pyocyanin biosynthesis in Pseudomonas aeruginosa PAO1.
Lundgren BR, Thornton W, Dornan MH, Villegas-Peñaranda LR, Boddy CN, Nomura CT
J. Bacteriol. 2013 May;195(9):2087-100
PubMed ID: 23457254
GcsR, a TyrR-Like Enhancer-Binding Protein, Regulates Expression of the Glycine Cleavage System in Pseudomonas aeruginosa PAO1.
Sarwar Z, Lundgren BR, Grassa MT, Wang MX, Gribble M, Moffat JF, Nomura CT
mSphere ;1(2):
PubMed ID: 27303730