Pseudomonas aeruginosa PAO1, PA2525 (opmB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2525
Name
opmB
Synonym: opmB
Replicon chromosome
Genomic location 2846283 - 2847779 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251215.1
GI 15597721
Affymetrix PA2525_at
Entrez 878719
GenBank AAG05913.1
INSDC AAG05913.1
NCBI Locus Tag PA2525
protein_id(GenBank) gb|AAG05913.1|AE004680_1|gnl|PseudoCAP|PA2525
TIGR NTL03PA02525
UniParc UPI0000001FD8
UniProtKB Acc Q9I0V8
UniProtKB ID Q9I0V8_PSEAE
UniRef100 UniRef100_G3XCM8
UniRef50 UniRef50_B2VFT0
UniRef90 UniRef90_G3XCM8

Product

Feature Type CDS
Coding Frame 1
Product Name
OpmB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.05
Kyte-Doolittle Hydrophobicity Value -0.238
Molecular Weight (kDa) 53.9
Isoelectric Point (pI) 5.11

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
OpmB OpmB is an outer membrane efflux protein in Pseudomonas aeruginosa that shows functional cooperation with MuxABC, to form the efflux pump system MuxABC-OpmB. [PMID:12445646] ARO:3004072 protein homolog model Perfect 943.3 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 334 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003040 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2525
Search term: opmB
Search term: OpmB

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Gene cloning and characteristics of the RND-type multidrug efflux pump MuxABC-OpmB possessing two RND components in Pseudomonas aeruginosa.
Mima T, Kohira N, Li Y, Sekiya H, Ogawa W, Kuroda T, Tsuchiya T
Microbiology (Reading) 2009 Nov;155(Pt 11):3509-3517
PubMed ID: 19713238