Pseudomonas aeruginosa PAO1, PA2527 (muxB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2527
Name
muxB
Synonym: yegN
Replicon chromosome
Genomic location 2850883 - 2854014 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 5 transposon mutants in orthologs

Cross-References

RefSeq NP_251217.1
GI 15597723
Affymetrix PA2527_at
Entrez 882889
GenBank AAG05915.1
INSDC AAG05915.1
NCBI Locus Tag PA2527
protein_id(GenBank) gb|AAG05915.1|AE004680_3|gnl|PseudoCAP|PA2527
TIGR NTL03PA02527
UniParc UPI00000C571E
UniProtKB Acc Q9I0V6
UniProtKB ID Q9I0V6_PSEAE
UniRef100 UniRef100_A0A069QAI5
UniRef50 UniRef50_Q1LR27
UniRef90 UniRef90_W0WFC0

Product

Feature Type CDS
Coding Frame 1
Product Name
MuxB
Product Name Confidence: Class 1
Synonyms probable RND efflux transporter
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.06
Kyte-Doolittle Hydrophobicity Value 0.355
Molecular Weight (kDa) 112.3
Isoelectric Point (pI) 5.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MuxB MuxB is one of the two necessary RND components in the Pseudomonas aeruginosa efflux pump system MuxABC-OpmB. [PMID:19713238] ARO:3004074 protein homolog model Perfect 1925.6 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 488 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001353 (1523 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2527
Search term: muxB
Search term: MuxB

Human Homologs

References

Gene cloning and characteristics of the RND-type multidrug efflux pump MuxABC-OpmB possessing two RND components in Pseudomonas aeruginosa.
Mima T, Kohira N, Li Y, Sekiya H, Ogawa W, Kuroda T, Tsuchiya T
Microbiology (Reading) 2009 Nov;155(Pt 11):3509-3517
PubMed ID: 19713238