Pseudomonas aeruginosa PAO1, PA2614 (lolA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 2956153 - 2956779 (- strand)


RefSeq NP_251304.1
GI 15597810
Affymetrix PA2614_lolA_at
DNASU PaCD00007127
Entrez 882320
GenBank AAG06002.1
INSDC AAG06002.1
NCBI Locus Tag PA2614
protein_id(GenBank) gb|AAG06002.1|AE004690_2|gnl|PseudoCAP|PA2614
UniParc UPI000012E7B5
UniProtKB Acc Q9I0M4
UniRef100 UniRef100_B7UW08
UniRef50 UniRef50_Q4K9V4
UniRef90 UniRef90_B7UW08


Feature Type CDS
Coding Frame 1
Product Name
lipoprotein localization protein LolA
Product Name Confidence: Class 1
Synonyms periplasmic chaperone LolA
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Charge (pH 7) -1.52
Kyte-Doolittle Hydrophobicity Value -0.154
Molecular Weight (kDa) 23.1
Isoelectric Point (pI) 5.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 1
Periplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2W7Q PROTEIN TRANSPORT 12/23/08 Structure of Pseudomonas aeruginosa LolA PSEUDOMONAS AERUGINOSA 1.88 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 204 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002937 (561 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2614
Search term: lolA


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Genetic analyses of the in vivo function of LolA, a periplasmic chaperone involved in the outer membrane localization of Escherichia coli lipoproteins.
Tajima T, Yokota N, Matsuyama S, Tokuda H
FEBS Lett. 1998 Nov;439(1-2):51-4
PubMed ID: 9849875
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011 ;2
PubMed ID: 21863142
Characterization of the Pseudomonas aeruginosa Lol system as a lipoprotein sorting mechanism.
Tanaka SY, Narita S, Tokuda H
J. Biol. Chem. 2007 May;282(18):13379-84
PubMed ID: 17350955