Pseudomonas aeruginosa PAO1, PA2771

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA2771
Name
Replicon	chromosome
Genomic location	3129729 - 3130754 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Comment	GGDEF motifs found in PAO1 and PACS2, but not PA14

Cross-References

RefSeq	NP_251461.1
GI	15597967
Affymetrix	PA2771_at
DNASU	PaCD00007204
Entrez	882750
GenBank	AAG06159.1
INSDC	AAG06159.1
NCBI Locus Tag	PA2771
protein_id(GenBank)	gb|AAG06159.1|AE004705_1|gnl|PseudoCAP|PA2771
TIGR	NTL03PA02771
UniParc	UPI00000C57F7
UniProtKB Acc	Q9I072
UniProtKB ID	Q9I072_PSEAE
UniRef100	UniRef100_Q9I072
UniRef50	UniRef50_Q9I072
UniRef90	UniRef90_Q9I072

Product

Feature Type	CDS
Coding Frame	1
Product Name	diguanylate cyclase with a self-blocked I-site, Dcsbis Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7)	-6.49
Kyte-Doolittle Hydrophobicity Value	-0.252
Molecular Weight (kDa)	38.0
Isoelectric Point (pI)	5.97

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543 Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
4ZMM	TRANSFERASE	05/04/15	GGDEF domain of Dcsbis complexed with c-di-GMP	Pseudomonas aeruginosa PAO1	2.503	X-RAY DIFFRACTION	99.5
4ZMU	LYASE	05/04/15	Dcsbis, a diguanylate cyclase from Pseudomonas aeruginosa	Pseudomonas aeruginosa PAO1	2.502	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 493 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG005129 (359 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA2771 Search term: diguanylate cyclase with a self-blocked I-site, Dcsbis

Human Homologs

References

Dcsbis (PA2771) from Pseudomonas aeruginosa is a highly active diguanylate cyclase with unique activity regulation. Chen Y, Liu S, Liu C, Huang Y, Chi K, Su T, Zhu D, Peng J, Xia Z, He J, Xu S, Hu W, Gu L Sci Rep 2016 Jul 8;6:29499 PubMed ID: 27388857