Pseudomonas aeruginosa PAO1, PA2771

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 3129729 - 3130754 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1


RefSeq NP_251461.1
GI 15597967
Affymetrix PA2771_at
DNASU PaCD00007204
Entrez 882750
GenBank AAG06159.1
INSDC AAG06159.1
NCBI Locus Tag PA2771
protein_id(GenBank) gb|AAG06159.1|AE004705_1|gnl|PseudoCAP|PA2771
UniParc UPI00000C57F7
UniProtKB Acc Q9I072
UniRef100 UniRef100_Q9I072
UniRef50 UniRef50_Q9I072
UniRef90 UniRef90_Q9I072


Feature Type CDS
Coding Frame 1
Product Name
diguanylate cyclase with a self-blocked I-site, Dcsbis
Product Name Confidence: Class 1
CommentGGDEF motifs found in PAO1 and PACS2, but not PA14
Evidence for Translation
Charge (pH 7) -6.49
Kyte-Doolittle Hydrophobicity Value -0.252
Molecular Weight (kDa) 38.0
Isoelectric Point (pI) 5.97

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4ZMM TRANSFERASE 05/04/15 GGDEF domain of Dcsbis complexed with c-di-GMP PSEUDOMONAS AERUGINOSA PAO1 2.503 X-RAY DIFFRACTION 99.5
4ZMU LYASE 05/04/15 Dcsbis, a diguanylate cyclase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA PAO1 2.502 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 493 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005129 (368 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2771

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 7024
Genomic Position 3130220
Position in ORF 492(1026)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_41_D2
Genomic Position 3130644
Position in ORF 916(1026)
Reference 15805499

Transposon Insertions in Orthologs


Dcsbis (PA2771) from Pseudomonas aeruginosa is a highly active diguanylate cyclase with unique activity regulation.
Chen Y, Liu S, Liu C, Huang Y, Chi K, Su T, Zhu D, Peng J, Xia Z, He J, Xu S, Hu W, Gu L
Sci Rep 2016 Jul;6
PubMed ID: 27388857